High expression of TIM-3 mRNA in urinary cells from kidney transplant recipients with acute rejection

High expression of TIM-3 mRNA in urinary cells from kidney transplant recipients with acute rejection

Author Renesto, P. G. Google Scholar
Ponciano, V. C. Google Scholar
Cenedeze, Marcos Antonio Autor UNIFESP Google Scholar
Camara, N. O. Saraiva Google Scholar
Pacheco-Silva, Alvaro Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract TIM-3 is a recently described molecule specifically expressed on Th1 differentiated T cells. We explored the usefulness of urinary mRNA profiles in the diagnosis of renal acute rejection (AR). Sixty urinary samples from renal transplant recipients simultaneously collected to allograft biopsy, (AR = 30 and No-AR =30), and 12 urinary samples from stable renal transplants were analyzed. Urinary mRNA encoding for TIM-3 and IFN-gamma were quantified using real time RT-PCR. TIM-3 mRNA was highly expressed in AR (559.19 +/- 644.41) compared to No-AR (3.78 +/- 7.20), and stable transplants (0.54 +/- 0.76), p < 0.001. To a lesser degree, IFN-gamma mRNA transcripts were also increased in AR (50.40 +/- 38.71), compared with No-AR (4.69 +/- 12.62), and stable transplants (0.38 +/- 0.44) p < 0.001. the highest expression of TIM-3 in AR makes it a promising noninvasive test for its diagnosis.
Keywords graft rejection
graft survival
immune monitoring
immune rejection
renal transplantation
TIM-3
Language English
Date 2007-06-01
Published in American Journal of Transplantation. Oxford: Blackwell Publishing, v. 7, n. 6, p. 1661-1665, 2007.
ISSN 1600-6135 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 1661-1665
Origin http://dx.doi.org/10.1111/j.1600-6143.2007.01795.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000246576700030
URI http://repositorio.unifesp.br/handle/11600/29771

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account