Absence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in rats

Absence of DNA damage in multiple organs (blood, liver, kidney, thyroid gland and urinary bladder) after acute fluoride exposure in rats

Author Leite, Aline de Lima Google Scholar
Santiago, Joel Ferreira Google Scholar
Levy, Flavia Mauad Google Scholar
Maria, Andrea Gutierrez Google Scholar
Fernandes, Mileni da Silva Google Scholar
Salvadori, Daisy Maria Favero Google Scholar
Ribeiro, Daniel Araki Autor UNIFESP Google Scholar
Rabelo Buzalaf, Marflia Afonso Google Scholar
Institution Universidade de São Paulo (USP)
Univ Estadual Paulista
Universidade Federal de São Paulo (UNIFESP)
Abstract Fluoride has been widely used in dentistry as a caries prophylactic agent. However, there has been some speculation that excess fluoride could cause an impact on genome integrity. in the current study, the potential DNA damage associated with exposure to fluoride was assessed in cells of blood, liver, kidney, thyroid gland and urinary bladder by the single cell gel (comet) assay. Male Wistar rats aging 75 days were distributed into seven groups: Groups 1 (control), 2, 3, 4, 5, 6 and 7 received 0 (deionized water), 10, 20, 40, 60, 80 and 100 mgF/Kg body weight from sodium fluoride (NaF), respectively, by gastrogavage. These groups were killed at 2 h after the administration of the fluoride doses. the level of DNA strand breaks did not increase in all organs evaluated and at all doses of NaF tested, as depicted by the mean tail moment. Taken together, our results suggest that oral exposure to NaF did not result in systemic genotoxic effect in multiple organs related to fluoride toxicity. Since DNA damage is an important step in events leading to carcinogenesis, this study represents a relevant contribution to the correct evaluation of the potential health risk associated with chemical exposure.
Keywords Acute exposure
Comet assay
DNA damage
Fluoride
Rats
Language English
Date 2007-05-01
Published in Human & Experimental Toxicology. London: Sage Publications Ltd, v. 26, n. 5, p. 435-440, 2007.
ISSN 0960-3271 (Sherpa/Romeo, impact factor)
Publisher Sage Publications Ltd
Extent 435-440
Origin https://dx.doi.org/10.1177/0960327107076288
Access rights Closed access
Type Article
Web of Science ID WOS:000248156000006
URI https://repositorio.unifesp.br/handle/11600/29743

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account