Antihypertensive effect of a standardized aqueous extract of Cecropia glaziovii Sneth in rats: An in vivo approach to the hypotensive mechanism

Antihypertensive effect of a standardized aqueous extract of Cecropia glaziovii Sneth in rats: An in vivo approach to the hypotensive mechanism

Author Lima-Landman, M. T. R. Google Scholar
Borges, A. C. R. Google Scholar
Cysneiros, Roberta Monterazzo Autor UNIFESP Google Scholar
De Lima, T. C. M. Google Scholar
Souccar, C. Google Scholar
Lapa, A. J. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Federal de Santa Catarina (UFSC)
Abstract Cecropia glaziovii Sneth is a common tree at the Southeastern Brazilian coast. As many other species of the genus, it shares the reputed folk use to treat heart failure, cough, asthma and bronchitis. the plant has been cultivated under controlled conditions and the 2% aqueous extract (AE) prepared with the dried leaves was standardized by its chemical contents on catechins, flavonoids and procyanidins. the present paper reports the antihypertensive activity of AE and of n-butanol fraction (BuF), an enriched semi-purified butanolic fraction used to isolate the main chemical constituents. Oral administration of AE and BuF induced hypotension in normotensive rats. the effect of AE (0.5 g/ kg/bi, p.o.) was time and dose-dependent peaking at 2-3 weeks after daily administration. BuF was faster but not more active than AE. Both extracts decreased the hypertension of spontaneous hypertensive rats, the hypertension induced in rats by L-NAME treatment and that induced by constriction of one renal artery. the antihypertensive effect was maintained for as long as 60 days of treatment and was reversible upon drug washout at the same rate of its establishment. Acute i.v. administration of BuF to anesthetized rats induced a fast short-lasting hypotension and inhibited the pressor responses to noradrenaline, angiotensin I and angiotensin II by 40%. These results were indirect indications that the hypotension induced by AE is not related to ACE inhibition, increased NO synthesis, or specific blockade of al and ATI receptors. It can be suggested that BuF interferes with the calcium handling mechanisms in smooth muscle cells and neurons. Intravenous injection of five out of nine compounds isolated from BuF produced immediate but short-lasting hypotension that does not correlate with the onset of the hypotension after oral treatment. This finding suggests that they may not be the compounds directly responsible for the delayed and sustained hypotension after per os administration of AE. the many compounds isolated from AE are under evaluation to determine its pharmacokinetics, mechanisms of action and interactions necessary to yield the plant effect. Although its mechanism is still unknown, AE seems to be an effective and safe anti hypertensive phytomedicine. (C) 2007 Elsevier GmbH. All rights reserved.
Keywords Cecropia glaziovii
medicinal plants
antihypertensive activity
phytomedicine
Language English
Date 2007-05-01
Published in Phytomedicine. Jena: Elsevier Gmbh, Urban & Fischer Verlag, v. 14, n. 5, p. 314-320, 2007.
ISSN 0944-7113 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 314-320
Origin http://dx.doi.org/10.1016/j.phymed.2007.03.003
Access rights Closed access
Type Article
Web of Science ID WOS:000247200100003
URI http://repositorio.unifesp.br/handle/11600/29713

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