Microarray cDNA to identify inflammatory genes in nasal polyposis

Microarray cDNA to identify inflammatory genes in nasal polyposis

Author Figueiredo, Claudia R. Google Scholar
Santos, Rodrigo P. Google Scholar
Silva, Ismael D. C. G. Google Scholar
Weckx, Luc L. M. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Background: the objective of this study was to investigate the spectrum of inflammatory gene expression in patients with nasal polyposis.Methods: the cDNA microarray technique was used to identify gene expression in tissue samples from nasal polyps and adjacent inflammatory nasal mucosa of 21 patients with nonallergic nasal polyposis. To validate the microarray analysis, we compared the expression of five genes by reverse transcription-polymerase chain reaction (RT-PCR): tumor necrosis factor, IL-5, IL-9, fibroblast growth factor, and transforming growth factor (TGF)-beta 1.Results: We tested 96 different inflammatory genes in our samples. Thirty-six genes exhibited differences in expression between the two tissue types. in all 36 genes the level of expression was greater in the inflammatory mucosa than the polyps. the RT-PCR confirmed the cDNA results.Conclusion: We believe that the high expression of TGF-beta 1 in inflammatory mucosa compared with the low expression in polyps may reflect an important role in the inhibitory mechanisms of nasal polyposis.
Keywords cDNA microarray
inflammatory genes
nasal polys etiology
nasal polyposis
nonallergic rhinitis
Language English
Date 2007-03-01
Published in American Journal of Rhinology. Providence: Ocean Side Publications Inc, v. 21, n. 2, p. 231-235, 2007.
ISSN 1050-6586 (Sherpa/Romeo, impact factor)
Publisher Ocean Side Publications Inc
Extent 231-235
Origin http://dx.doi.org/10.2500/ajr.2007.21.2968
Access rights Closed access
Type Article
Web of Science ID WOS:000245563700020
URI http://repositorio.unifesp.br/handle/11600/29587

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