The effects of soy extract on the uterus of castrated adult rats

The effects of soy extract on the uterus of castrated adult rats

Author Mosquette, Rejane Google Scholar
Simoes, Manuel de Jesus Google Scholar
Silva, Ismael Dale Cotrim Guerreiro da Autor UNIFESP Google Scholar
Oshima, Celina Tizuko Fujiyama Autor UNIFESP Google Scholar
Oliveira-Filho, Ricardo Martins Google Scholar
Haidar, Mauro Abi Autor UNIFESP Google Scholar
Simoes, Ricardo Santos Google Scholar
Baracat, Edmund Chada Autor UNIFESP Google Scholar
Soares Junior, Jose Maria Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Objective: the aim of this study was to evaluate the effects of different doses of a standardized soy extract on the uterus of castrated rats.Methods: Fifty-six adult castrated female Wistar rats were randomly divided into seven groups (eight animals in each) that received: GI-drug vehicle (propylene glycol); GII-soy extract 10mg/kg per day; GIII-soy extract 50mg/kg per day; GIV-soy extract 100mg/kg per day; GV-soy extract 300mg/kg per day; GVI-soy extract 600mg/kg per day; GVII-conjugated equine estrogens (CEE) 200 mu g/kg per day. After 21 days of treatment, all animals were sacrificed and fragments of the uterine horns were immediately removed, fixed in 10% formaldehyde and submitted to routine histological techniques for morphometric study. the endometrial cell proliferation index was determined with the PCNA antibody PC-10 and expressed as the percentuals of the PCNA-positive nuclei relative to the total countings. Other fragments were immediately frozen in liquid nitrogen for RNA extraction and VEGF analysis using RT-PCR technique.Results: the minimal dose of soy extract that produced a significant increase of the morphometric parameters was 100 mg/kg (GIV). the maximum effects on endometrial and myometrial morphometry were detected in the groups treated with 300 and 600 mg/kg of soy extract (groups V and VI) and CEE (GVII). the expression of PCNA in the endometrial epithelium and stroma was increased by treatment with 100-600 mg/kg per day of soy extract (groups IV-VI) or with CCE (group VII). Doses equal to or higher than 50 mg/kg of soy extract (groups III-VI) and CEE stimulated the expression of VEGF.Conclusion: the treatment of adult castrated rats during 21 days with doses of 100 mg/kg per day or higher of soy extract may determine significant proliferation in the endometrium and myometrium. (c) 2006 Published by Elsevier Ireland Ltd.
Keywords soy extract
isoflavones
castrated rat
uterus
VEGF
morphology
endometrium
Language English
Date 2007-02-20
Published in Maturitas. Clare: Elsevier B.V., v. 56, n. 2, p. 173-183, 2007.
ISSN 0378-5122 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 173-183
Origin http://dx.doi.org/10.1016/j.maturitas.2006.07.011
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000244156700008
URI http://repositorio.unifesp.br/handle/11600/29513

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