Europium tetracycline biosensor for the determination of cholesterol - art. no. 643017

Europium tetracycline biosensor for the determination of cholesterol - art. no. 643017

Author Courrol, Lilia Coronato Google Scholar
Silva, Flávia Rodrigues de Oliveira Google Scholar
Samad, Ricardo Elgul Google Scholar
Mansano, Ronaldo Domingues Google Scholar
Vieira, Nilson Dias Google Scholar
VoDinh, T. Google Scholar
Grundfest, W. S. Google Scholar
Benaron, D. A. Google Scholar
Cohn, G. E. Google Scholar
Raghavachari, R. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Development of cholesterol biosensors is of great importance in clinical analysis because the concentration of cholesterol in blood is a fundamental parameter for the prevention and diagnosis of a number of clinical disorders such as heart disease, hypertension and arteriosclerosis. in general, determination of cholesterol is based on spectrophotometry; but this method involves complicated procedures and the cost is high because expensive enzyme must be used in each assay.We report here the observation, for the first time, of the enhancement of Europium-Tetracycline complex emission in cholesterol solutions. This enhancement was initially observed with the addition of the enzyme cholesterol oxidase, which produces H2O2, the agent driver of the Europium tetracycline complex, to the solution. However, it was found that the enzyme is not needed to enhance the luminescence. A calibration curve was determined, resulting in an easy-handling immobilization method with a cheap stable material. This method shows that the complex can be used as a sensor to determine cholesterol in biological systems with good selectivity, fast response, miniature size, and reproducible results.
Keywords cholesterol
Europium
analytical analysis
Language English
Date 2007-01-01
Published in Advanced Biomedical and Clinical Diagnostic Systems V. Bellingham: Spie-int Soc Optical Engineering, v. 6430, p. 43017-43017, 2007.
ISSN 0277-786X (Sherpa/Romeo, impact factor)
Publisher Spie-int Soc Optical Engineering
Extent 43017-43017
Origin http://dx.doi.org/10.1117/12.697956
Access rights Closed access
Type Conference paper
Web of Science ID WOS:000245947500024
URI http://repositorio.unifesp.br/handle/11600/29430

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