Investigation of chromosome 21 aneuploidies in breast fibroadenomas by fluorescence in situ hybridisation

Investigation of chromosome 21 aneuploidies in breast fibroadenomas by fluorescence in situ hybridisation

Author Leite, D. Soares Google Scholar
Lima, P. D. Lima de Google Scholar
Leal, M. Ferreira Google Scholar
Chen, E. Suchi Google Scholar
Casartelli, C. Google Scholar
Cardoso Smith, M. de Arruda Google Scholar
Burbano, R. Rodriguez Google Scholar
Institution Fed Univ Para
Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract Fibroadenoma (FA) is a benign breast tumour that occurs in about 25% of women. Cytogenetic studies suggest that numerical chromosomal aberrations may contribute to tumorigenesis, but chromosomal instability is still poorly characterised in breast cancer. the aim of this study was to investigate numerical alterations of chromosome 21 in 15 breast FAs. All samples were analysed by classical cytogenetics and by fluorescence in situ hybridisation (FISH) for chromosome 21 DNA sequences. Classical cytogenetics analysis showed that all cells were diploidies with modal number varying between 43 and 47 chromosomes, and clonal chromosome alterations in 46.7% of tumours. Clonal numerical alterations involved, preferentially, chromosomes 8, 10, 12, 16 and 21. FISH analysis showed a statistically significant difference for chromosome 21 monosomy between seven samples and control group. This monosomy varied from 24.5% to 43.5% of analysed cells. the presence of chromosomal alterations in FAs may be a consequence of the proliferation process and is probably not related to the aetiology of this type of lesion. the study of benign proliferations and comparison with chromosome alterations in their malignant counterparts should result in an understanding of the genes acting in cell proliferation alone and those that cause these cells to both undergo malignant transformation and become invasive.
Keywords fibroadenoma
breast tumour
chromosome 21
aneuploidy
monosomy fluorescence in situ hybridisation
Language English
Date 2006-12-01
Published in Clinical and Experimental Medicine. New York: Springer, v. 6, n. 4, p. 166-170, 2006.
ISSN 1591-8890 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 166-170
Origin http://dx.doi.org/10.1007/s10238-006-0112-9
Access rights Closed access
Type Article
Web of Science ID WOS:000243226700004
URI http://repositorio.unifesp.br/handle/11600/29278

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