Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa

Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa

Author Cedenho, Agnaldo Pereira Autor UNIFESP Google Scholar
Lima, Samira Barbosa Autor UNIFESP Google Scholar
Cenedeze, Marcos Antonio Autor UNIFESP Google Scholar
Spaine, Deborah Montagnini Autor UNIFESP Google Scholar
Ortiz, Valdemar Autor UNIFESP Google Scholar
Oehninger, S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Jones Inst Reprod Med
Abstract BACKGROUND: Heat-shock protein A2 (HspA2) is correlated with sperm maturity, function and fertility, and a dysfunctional expression of such a gene results in abnormal spermatogenesis. the purpose of this study was to compare HspA2 gene expression in spermatozoa from oligozoospermic men and normozoospermic controls. METHODS: Semen was obtained and analysed according to World Health Organization (World Health Organization, 1999) guidelines, morphology by Kruger's strict criteria. Seventeen patients with oligozoospermia and 21 fertile controls were studied. Total RNA was extracted from ejaculated and Percoll density-gradient-separated spermatozoa followed by semiquantitative RT-PCR analysis. the relative expression level of HspA2 was analysed according to the expression level of the housekeeping beta-actin gene. Serum hormonal profiles (FSH, LH and testosterone) and a peripheral karyotype were also performed. RESULTS: All patients possessed normal karyotype, and no significant hormonal differences were found between the two groups. the study group had significantly lower sperm concentration and normal morphology than the controls. Semiquantitative RT-PCR analysis of HspA2 showed significantly lower expression levels in the oligoteratozoospermic men when compared to controls (P = 0.0021). CONCLUSIONS: the HspA2 gene was down-regulated in sperm from infertile men with idiopathic oligoteratozoospermia, suggesting that such anomalies of gene expression might be associated with pathogenesis in some subtypes of male infertility.
Keywords gene expression
male infertility
Language English
Date 2006-07-01
Published in Human Reproduction. Oxford: Oxford Univ Press, v. 21, n. 7, p. 1791-1794, 2006.
ISSN 0268-1161 (Sherpa/Romeo, impact factor)
Publisher Oxford Univ Press
Extent 1791-1794
Origin http://dx.doi.org/10.1093/humrep/del055
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000238907400021
URI http://repositorio.unifesp.br/handle/11600/28989

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