Repeated electroconvulsive shock induces changes in high-affinity [H-3]-ouabain binding to rat striatal membranes

Repeated electroconvulsive shock induces changes in high-affinity [H-3]-ouabain binding to rat striatal membranes

Author Bignotto, Magda Autor UNIFESP Google Scholar
Venditti, Marco Antonio Campana Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Repeated electroconvulsive shock is an effective treatment for affective disorders. Striatum, hippocampus and brainstem are involved in affective disorders. Sodium-potassium/ATPase is of paramount importance for the proper functioning of the brain and its involvement in the affective disorders has been claimed for a long time. Sodium-potassium/ATPase has an extracellular regulatory binding site to which cardiotonic glycosides, such as ouabain, bind to, thus regulating the activity of the enzyme. Endogenous ouabain-like substances exist in the brain and their actions on the sodium-potassium/ATPase resemble ouabain biological properties. the aim of this work was to determine if electroconvulsive shock (ECS) would induce changes in the high-affinity binding of ouabain to the sodium-potassium/ATPase from rat brain regions. Adult, male Wistar rats received one (ECSx1 group) or seven electroshocks (ECSx7 group) delivered daily through ear-clips electrodes. Control rats received the same manipulations; however, no current was delivered through the electrodes (SHAMx1 and SHAMx7 groups). All groups were sacrificed 24 h after the last ECS session. the B-max and K-D of high-affinity [3H]-ouabain binding were determined in crude membrane preparations from the striatum, hippocampus and brainstem. the results obtained showed a statistically significant increase in the affinity of [H-3]-ouabain (lower K (D)) to striatal membranes in those rats receiving seven ECS. in the striatum there was no change in the K (D) after one ECS; as well as there was no change in the B-max after a single or seven ECS. High-affinity [H-3]-ouabain binding to hippocampus and brainstem did not reveal any significant differences either in K (D) or B-max after one or seven ECS. the increased affinity of ouabain to the striatal sodium-potassium/ATPase induced by repeated ECS suggests an increased interaction in vivo of the endogenous ouabain-like substances with the enzyme and the involvement of the extracellular regulatory allosteric ouabain binding site in the striatal sodium-potassium/ATPase in the effects of electroconvulsive shock.
Keywords electroconvulsive shock
[H-3]-ouabain
sodium-potassium/ATPase
striatum
hippocampus
brainstem
Language English
Date 2006-04-01
Published in Neurochemical Research. New York: Springer/plenum Publishers, v. 31, n. 4, p. 515-521, 2006.
ISSN 0364-3190 (Sherpa/Romeo, impact factor)
Publisher Springer
Extent 515-521
Origin http://dx.doi.org/10.1007/s11064-006-9046-6
Access rights Closed access
Type Article
Web of Science ID WOS:000238138800009
URI http://repositorio.unifesp.br/handle/11600/28808

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