N-RAS and K-RAS gene mutations in Brazilian patients with multiple myeloma

N-RAS and K-RAS gene mutations in Brazilian patients with multiple myeloma

Author Ortega, Manoela M. Google Scholar
Faria, Rosa MD Autor UNIFESP Google Scholar
Shitara, Edson S. Google Scholar
Assis, Angela M. Google Scholar
Albuquerque, Dulcinéia M. Google Scholar
Oliveira, José SR Google Scholar
Noguti, Maria Aparecida Eiko Autor UNIFESP Google Scholar
Faria, Jose Roberto de Autor UNIFESP Google Scholar
Costa, Fernando Ferreira Autor UNIFESP Google Scholar
Lima, Carmen Silvia Passos Google Scholar
Institution Universidade Estadual de Campinas (UNICAMP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Point mutations affecting codons 12, 13 (exon 1) and 61 (exon 2) of the N-RAS gene and codons 12 and 13 (exon 1) of the K-RAS gene are identified in approximately 30.0% and 10.0%, respectively, of multiple myeloma (MM) patients living in the northern hemisphere. To date, there are no reports about the prevalence of RAS gene mutations in MM Brazilian patients, and this comprised the aim of the present study. DNA from bone marrow aspirates of 252 patients with MM (139 males and 113 females; aged 59.33 +/- 11.95 years) were investigated for whole exons 1 and 2 of the N-RAS gene and whole exon 1 of the K-RAS gene by direct sequencing of DNA amplified in vitro by the polymerase chain reaction. Fifty-three out of 252 (21.03%) MM patients presented RAS mutations. Heterozygous mutations at codons 4, 10 (exon 1), 61 and 65 (exon 2) of the N - RAS gene were identified in seven out of 252 (2.78%) patients. K-RAS heterozygous mutations at codons 7, 12, 13 (exon 1) were seen in 46 out of 252 (18.25%) patients. To the best of our knowledge, the mutation at codon 7 of K-RAS gene is reported for the first time in MM. Taken together, these results suggest that Brazilian MM patients are characterized by: (i) a low prevalence of RAS mutation and (ii) RAS mutations located at distinct regions of the critical codons of the N - RAS and K-RAS genes.
Keywords multiple myeloma
mutation
N-RAS
K-RAS
Language English
Date 2006-02-01
Published in Leukemia & Lymphoma. Abingdon: Taylor & Francis Ltd, v. 47, n. 2, p. 285-289, 2006.
ISSN 1042-8194 (Sherpa/Romeo, impact factor)
Publisher Taylor & Francis Ltd
Extent 285-289
Origin http://dx.doi.org/10.1080/10428190500300969
Access rights Closed access
Type Article
Web of Science ID WOS:000233641400012
URI http://repositorio.unifesp.br/handle/11600/28731

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