Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections

Application of four molecular typing methods for analysis of Mycobacterium fortuitum group strains causing post-mammaplasty infections

Author Sampaio, JLM Autor UNIFESP Google Scholar
Chimara, Erica Google Scholar
Ferrazoli, Lucilaine Google Scholar
Telles, Maria Alice da Silva Google Scholar
Del Guercio, VMF Google Scholar
Jerico, ZVN Google Scholar
Miyashiro, Kozue Autor UNIFESP Google Scholar
Fortaleza, CMCB Google Scholar
Padoveze, M. C. Google Scholar
Leao, S. C. Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Fleury Ctr Med Diagnost
Inst Adolfo Lutz Registro
Inst Vozza Med & Diagnose LTDA
Ctr Vigilancia Epidemiol Prof Alexandre Vranjac
Abstract A cluster of cases of post-augmentation mammaplasty surgical site infections occurred between 2002 and 2004 in Campinas, in the southern region of Brazil. Rapidly growing mycobacteria were isolated from samples from 12 patients. Eleven isolates were identified as Mycobacterium fortuitum and one as Mycobacterium porcinum by PCR-restriction digestion of the hsp65 gene. These 12 isolates, plus six additional M. fortuitum isolates from non-related patients, were typed by pulsed-field gel electrophoresis (PFGE) and three PCR-based techniques: 16S-23S rRNA internal transcribed spacer (ITS) genotyping; randomly amplified polymorphic DNA (RAPD) PCR; and enterobacterial repetitive intergenic consensus (ERIC) PCR. Four novel M. fortuitum allelic variants were identified by restriction analysis of the ITS fragment. One major cluster, comprising six M. fortuitum isolates, and a second cluster of two isolates, were identified by the four methods. RAPD-PCR and ITS genotyping were less discriminative than ERIC-PCR. ERIC-PCR was comparable to PFGE as a valuable complementary tool for investigation of this type of outbreak.
Keywords infections
mammaplasty
molecular epidemiology
Mycobacterium fortuitum
plastic surgery
typing
Language English
Date 2006-02-01
Published in Clinical Microbiology and Infection. Oxford: Blackwell Publishing, v. 12, n. 2, p. 142-149, 2006.
ISSN 1198-743X (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing
Extent 142-149
Origin http://dx.doi.org/10.1111/j.1469-0691.2005.01312.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000234698700008
URI http://repositorio.unifesp.br/handle/11600/28709

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