Apoptotic effect of ethanol is potentiated by caffeine-induced calcium release in rat astrocytes

Apoptotic effect of ethanol is potentiated by caffeine-induced calcium release in rat astrocytes

Author Hirata, H. Google Scholar
Machado, L. S. Google Scholar
Okuno, C. S. Google Scholar
Brasolin, A. Google Scholar
Lopes, G. S. Google Scholar
Smaili, Soraya Soubhi Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract In this study, we investigated agents that increased intracellular calcium levels and their correlation with apoptotic cell death induction. We used rat astrocytes to investigate the increase in cytosolic Ca2+, (Ca-c(2+)) and apoptosis induction by drugs that mobilize Ca2+ from different sources. We observed that thapsigargin (Thap), caffeine (Caff) and FCCP which caused similar increases in Ca-c(2+) levels (30-40%), also induced similar apoptotic rates (30-35%). On the other hand, antimycin (Anti), staurosporine (STS) and ethanol (Eth) promoted higher increases in Ca-c(2+) (55-65 %) and higher apoptotic rates (55-85%). Eth induced cell death in a concentration- and time-dependent manner. After treatment with Eth plus Caff for 6, 12 and 24 h, these effects were strongly potentiated. Results suggest that there might be a correlation between Ca-c(2+) increase and the rate of apoptosis. It is possible that Eth induces cell death by activation of more than one pathway and Ca-c(2+) might be one of the elements involved. the present work indicates that Ca-c(2+) can potentiate death by ethanol in rat astrocytes. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
Keywords ethanol
calcium
mitochondria
apoptosis
astrocytes
endoplasmic reticulum
Language English
Date 2006-01-30
Published in Neuroscience Letters. Clare: Elsevier B.V., v. 393, n. 2-3, p. 136-140, 2006.
ISSN 0304-3940 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 136-140
Origin http://dx.doi.org/10.1016/j.neulet.2005.09.066
Access rights Closed access
Type Article
Web of Science ID WOS:000234583000012
URI http://repositorio.unifesp.br/handle/11600/28695

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