Adaptive expression pattern of different proteins involved in cellular calcium homeostasis in denervated rat vas deferens

Adaptive expression pattern of different proteins involved in cellular calcium homeostasis in denervated rat vas deferens

Author Quintas, LEM Google Scholar
Cunha, VMN Google Scholar
Scaramello, CBV Google Scholar
Silva, CLM da Google Scholar
Caricati-Neto, A. Google Scholar
Lafayette, SSL Google Scholar
Jurkiewicz, A. Google Scholar
Noel, F. Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Abstract The activity and protein expression of plasma membrane and sarco(endo)plasmic reticulum (Ca2+-Mg2+)ATPases and ryanodine receptors were investigated in surgically denervated rat vas deferens. the function of thapsigargin-sensitive but not thapsigargin-resistant (Ca2+-Mg2+) ATPase (from sarco(endo)plasmic reticulum and plasma membrane, respectively), evidenced by enzyme activity and Ca2+ uptake experiments, was significantly depressed by 30-50% when compared to innervated vas. Western blots showed that such reduction in sarco(endo)plasmic reticulum (Ca2+-Mg2+)ATPase performance was accompanied by a decrement of similar magnitude in sarco(endo)plasmic reticulum (Ca2+-Mg2+) ATPase type 2 protein expression, without any significant change in plasma membrane (Ca2+-Mg2+)ATPase expression. Finally, [3 H]ryanodine binding revealed that the density of ryanodine binding sites was reduced by 45% after denervation without modification in affinity. the present findings demonstrate that sarco(endo)plasmic reticulum proteins involved in intracellular calcium homeostasis are clearly down-regulated and brings further evidence of a modified calcium translocation in denervated rat vas deferens. (c) 2005 Elsevier B.V All rights reserved.
Keywords denervation
vas deferens
sarco(endo)plasmic reticulum (Ca2+-Mg2+)ATPase
plasma membrane (Ca2+-Mg2+)ATPase
ryanodine receptor
calcium
Language English
Date 2005-11-21
Published in European Journal of Pharmacology. Amsterdam: Elsevier B.V., v. 525, n. 1-3, p. 54-59, 2005.
ISSN 0014-2999 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 54-59
Origin http://dx.doi.org/10.1016/j.ejphar.2005.10.006
Access rights Closed access
Type Article
Web of Science ID WOS:000233711400008
URI http://repositorio.unifesp.br/handle/11600/28555

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