Glycomics analyses of tear fluid for the diagnostic detection of ocular rosacea

Glycomics analyses of tear fluid for the diagnostic detection of ocular rosacea

Author An, H. J. Google Scholar
Ninonuevo, M. Google Scholar
Aguilan, J. Google Scholar
Liu, H. Google Scholar
Lebrilla, C. B. Google Scholar
Alvarenga, L. S. Google Scholar
Mannis, M. J. Google Scholar
Institution Univ Calif Davis
Universidade Federal de São Paulo (UNIFESP)
Abstract A Glycomics approach to detect disease is illustrated in the analyses of human tear fluid for rosacea. the diagnosis of ocular rosacea is particularly challenging in a subgroup of patients that do not present with typical facial skin findings but have ocular signs and symptoms. Indeed, up to 90% of patients with ocular rosacea may have neither obvious roseatic skin changes nor a previous diagnosis of rosacea. Tear fluid was collected from 37 subjects (21 controls and 16 patients with ocular rosacea) after conjunctival stimulation with filter (Schirmer) paper. O-linked oligosaccharides were released from tear fluid by beta-elimination and then purified using solid-phase extraction. Mass spectra were recorded on an external source HiResMALDI with a 7.0 T magnet. Mass spectra were obtained in both the positive and negative modes. However, signals were stronger in the negative mode. Tear fluid samples from rosacea patients yielded distinctive clusters of peaks that extend to higher masses. Patients with rosacea presented several oligomeric series that were not found in the controls. To discriminate the ocular rosacea cases from the normal controls, the sum of absolute intensities of 13 series corresponding to nearly 50 identified mass spectrum peaks was used. Thirty-six out of the 37 samples were correctly classified. This yields a sensitivity of 100% (95% CI 79.5-100) and specificity of 95.2% (95% CI 76.299.9). the high abundance of oligosaccharides in the tear fluid of patients with rosacea may lead to an objective diagnostic marker for the disease.
Keywords ocular rosacea
glycomics
mucin
O-linked oligosaccharides
MALDI-FTMS
biomarker
Language English
Date 2005-11-01
Published in Journal of Proteome Research. Washington: Amer Chemical Soc, v. 4, n. 6, p. 1981-1987, 2005.
ISSN 1535-3893 (Sherpa/Romeo, impact factor)
Publisher Amer Chemical Soc
Extent 1981-1987
Origin http://dx.doi.org/10.1021/pr0501620
Access rights Closed access
Type Article
Web of Science ID WOS:000234007200009
URI http://repositorio.unifesp.br/handle/11600/28533

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