Characterization of an ecto-ATPase activity in Cryptococcus neoformans

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dc.contributor.author Junior, I. C.
dc.contributor.author Rodrigues, M. L.
dc.contributor.author Alviano, C. S.
dc.contributor.author Travassos, Luiz Rodolpho [UNIFESP]
dc.contributor.author Meyer-Fernandes, JR
dc.date.accessioned 2016-01-24T12:37:55Z
dc.date.available 2016-01-24T12:37:55Z
dc.date.issued 2005-07-01
dc.identifier http://dx.doi.org/10.1016/j.femsyr.2005.04.005
dc.identifier.citation Fems Yeast Research. Amsterdam: Elsevier B.V., v. 5, n. 10, p. 899-907, 2005.
dc.identifier.issn 1567-1356
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28351
dc.description.abstract Cryptococcus neoformans is the causative agent of pulmonary cryptococcosis and cryptococcal meningoencephalitis, which are major clinical manifestations in immunosuppressed patients. in the present study, a surface ATPase (ecto-ATPase) was identified in C. neoformans yeast cells. Intact yeasts hydrolyzed adenosine-5'-triphosphate (ATP) at a rate of 29.36 +/- 3.36 nmol Pi/h x 10(8) cells. in the presence of 5 mM MgCl2, this activity was enhanced around 70 times, and an apparent K-m for Mg-ATP corresponding to 0.61 mM was determined. Inhibitors of phosphatases, mitochondrial Mg2+-ATPases, V-ATPases, Na+-ATPases or P-ATPases had no effect on the cryptococcal ATPase, but extracellular impermeant compounds reduced enzyme activity in living cells. ATP was the best substrate for the cryptococcal ecto-enzyme, but it also efficiently hydrolyzed inosine 5'-triphosphate (ITP), cytidine 5'-triphosphate (CTP), guanosine 5'-triphosphate (GTP) and uridine-5'-triphosphate (UTP). in the presence of ATP, C. neoformans became less susceptible to the antifungal action of fluconazole. Our results are indicative of the occurrence of a C. neoformans ectoATPase that may have a role in fungal physiology. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved. en
dc.format.extent 899-907
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Fems Yeast Research
dc.rights Acesso aberto
dc.subject Cryptococcus neoformans en
dc.subject Ecto-ATPase en
dc.subject antifungal en
dc.title Characterization of an ecto-ATPase activity in Cryptococcus neoformans en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal do Rio de Janeiro (UFRJ)
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Univ Fed Rio de Janeiro, Inst Bioquim Med, CCS, BR-21541590 Rio de Janeiro, Brazil
dc.description.affiliation Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo Goes, CCS, BR-21541590 Rio de Janeiro, Brazil
dc.description.affiliation UNIFESP, Disciplina Biol Celular, São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, Disciplina Biol Celular, São Paulo, Brazil
dc.identifier.doi 10.1016/j.femsyr.2005.04.005
dc.description.source Web of Science
dc.identifier.wos WOS:000230688800002



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