Characterization of an ecto-ATPase activity in Cryptococcus neoformans

Characterization of an ecto-ATPase activity in Cryptococcus neoformans

Author Junior, I. C. Google Scholar
Rodrigues, M. L. Google Scholar
Alviano, C. S. Google Scholar
Travassos, Luiz Rodolpho Autor UNIFESP Google Scholar
Meyer-Fernandes, JR Google Scholar
Institution Universidade Federal do Rio de Janeiro (UFRJ)
Universidade Federal de São Paulo (UNIFESP)
Abstract Cryptococcus neoformans is the causative agent of pulmonary cryptococcosis and cryptococcal meningoencephalitis, which are major clinical manifestations in immunosuppressed patients. in the present study, a surface ATPase (ecto-ATPase) was identified in C. neoformans yeast cells. Intact yeasts hydrolyzed adenosine-5'-triphosphate (ATP) at a rate of 29.36 +/- 3.36 nmol Pi/h x 10(8) cells. in the presence of 5 mM MgCl2, this activity was enhanced around 70 times, and an apparent K-m for Mg-ATP corresponding to 0.61 mM was determined. Inhibitors of phosphatases, mitochondrial Mg2+-ATPases, V-ATPases, Na+-ATPases or P-ATPases had no effect on the cryptococcal ATPase, but extracellular impermeant compounds reduced enzyme activity in living cells. ATP was the best substrate for the cryptococcal ecto-enzyme, but it also efficiently hydrolyzed inosine 5'-triphosphate (ITP), cytidine 5'-triphosphate (CTP), guanosine 5'-triphosphate (GTP) and uridine-5'-triphosphate (UTP). in the presence of ATP, C. neoformans became less susceptible to the antifungal action of fluconazole. Our results are indicative of the occurrence of a C. neoformans ectoATPase that may have a role in fungal physiology. (c) 2005 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Keywords Cryptococcus neoformans
Ecto-ATPase
antifungal
Language English
Date 2005-07-01
Published in Fems Yeast Research. Amsterdam: Elsevier B.V., v. 5, n. 10, p. 899-907, 2005.
ISSN 1567-1356 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 899-907
Origin http://dx.doi.org/10.1016/j.femsyr.2005.04.005
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000230688800002
URI http://repositorio.unifesp.br/handle/11600/28351

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