Ultrastructural, immunohistochemical and biochemical analysis of glycosarninoglycans and proteoglycans in the mouse, pubic symphysis during pregnancy

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dc.contributor.author Pinheiro, M. C.
dc.contributor.author Mora, O. A.
dc.contributor.author Caldini, E. G.
dc.contributor.author Battlehner, C. N.
dc.contributor.author Joazeiro, P. P.
dc.contributor.author Toledo, OMS
dc.date.accessioned 2016-01-24T12:37:53Z
dc.date.available 2016-01-24T12:37:53Z
dc.date.issued 2005-06-01
dc.identifier http://dx.doi.org/10.1016/j.cellbi.2004.11.025
dc.identifier.citation Cell Biology International. London: Academic Press Ltd Elsevier B.V., v. 29, n. 6, p. 458-471, 2005.
dc.identifier.issn 1065-6995
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/28323
dc.description.abstract During pregnancy, an interpubic ligament is formed in the mouse pubic symphysis, in late stages, this ligament undergoes relaxation to allow proper delivery, which is expected on the 19th day, Proteoglycans and hyaluronic acid play an important role in the remodeling of the extracelfular matrix in these tissues. Gl cosarilinogl cans and protcogl cans were studied by electron microscopic, immunohistochemical and biochemical methods in samples of mouse pubic symphysis from the 12th to 18th day of pregnancy.At the ultrastructural level, using cuprolinic blue and enzymatic digestion by chondroitin lyases, two types of proteoglycan filaments were observed in the fibrocartilage on the 12th day, as well Lis in D15, D17 and D18 pubic ligaments. the only sulfated glycosaminoglycan in these filaments was chondroitin sulfate. as shown by chondroitin lyase treatment. Their electrophoretic mobility, before and after enzymatic degradation. corroborated this inference, the ratio of chondroitin Sulfate dry weight of symphysis showed two phases of increase: between D12 and D15. and between D17 and D18 WC Suggest that the first corresponds mainly to an increase in decorin when the ligament is formed, and the second to versican, during relaxation. Versican and hyaluronic acid. working as water holding molecules would be responsible for the hydration of the ligament at the end of pregnancy, allowing an increase in resiliency. the presence of hyaluronic acid was confirmed by labeling with HA-probe in the perichondrium, fibrocartilage and ligament. the role of collagen fibers as physical restrictors of the complete expansion of glycosaminoglycans and hyaluronic acid in tissue is discussed. (c) 2005 International Federation for Cell Biology. Published by Elsevier B.V. All rights reserved. en
dc.format.extent 458-471
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Cell Biology International
dc.rights Acesso restrito
dc.subject proteoglycan en
dc.subject hyaluronic acid en
dc.subject pubic symphysis en
dc.subject pregnancy en
dc.title Ultrastructural, immunohistochemical and biochemical analysis of glycosarninoglycans and proteoglycans in the mouse, pubic symphysis during pregnancy en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Universidade Estadual de Campinas (UNICAMP)
dc.contributor.institution Universidade de São Paulo (USP)
dc.description.affiliation UNIFESP, Discipline Hist & Struct Biol, Dept Morphol, BR-04023062 São Paulo, Brazil
dc.description.affiliation UNICAMP, Inst Biol, Dept Cell Biol, São Paulo, Brazil
dc.description.affiliation Univ São Paulo, Sch Med, Dept Pathol, Lab Cell Biol, São Paulo, Brazil
dc.description.affiliation UNICAMP, Inst Biol, Dept Histol & Embryol, São Paulo, Brazil
dc.description.affiliationUnifesp UNIFESP, Discipline Hist & Struct Biol, Dept Morphol, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1016/j.cellbi.2004.11.025
dc.description.source Web of Science
dc.identifier.wos WOS:000231189200008



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