Ultrastructural, immunohistochemical and biochemical analysis of glycosarninoglycans and proteoglycans in the mouse, pubic symphysis during pregnancy

Ultrastructural, immunohistochemical and biochemical analysis of glycosarninoglycans and proteoglycans in the mouse, pubic symphysis during pregnancy

Author Pinheiro, M. C. Google Scholar
Mora, O. A. Google Scholar
Caldini, E. G. Google Scholar
Battlehner, C. N. Google Scholar
Joazeiro, P. P. Google Scholar
Toledo, OMS Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
Abstract During pregnancy, an interpubic ligament is formed in the mouse pubic symphysis, in late stages, this ligament undergoes relaxation to allow proper delivery, which is expected on the 19th day, Proteoglycans and hyaluronic acid play an important role in the remodeling of the extracelfular matrix in these tissues. Gl cosarilinogl cans and protcogl cans were studied by electron microscopic, immunohistochemical and biochemical methods in samples of mouse pubic symphysis from the 12th to 18th day of pregnancy.At the ultrastructural level, using cuprolinic blue and enzymatic digestion by chondroitin lyases, two types of proteoglycan filaments were observed in the fibrocartilage on the 12th day, as well Lis in D15, D17 and D18 pubic ligaments. the only sulfated glycosaminoglycan in these filaments was chondroitin sulfate. as shown by chondroitin lyase treatment. Their electrophoretic mobility, before and after enzymatic degradation. corroborated this inference, the ratio of chondroitin Sulfate dry weight of symphysis showed two phases of increase: between D12 and D15. and between D17 and D18 WC Suggest that the first corresponds mainly to an increase in decorin when the ligament is formed, and the second to versican, during relaxation. Versican and hyaluronic acid. working as water holding molecules would be responsible for the hydration of the ligament at the end of pregnancy, allowing an increase in resiliency. the presence of hyaluronic acid was confirmed by labeling with HA-probe in the perichondrium, fibrocartilage and ligament. the role of collagen fibers as physical restrictors of the complete expansion of glycosaminoglycans and hyaluronic acid in tissue is discussed. (c) 2005 International Federation for Cell Biology. Published by Elsevier B.V. All rights reserved.
Keywords proteoglycan
hyaluronic acid
pubic symphysis
pregnancy
Language English
Date 2005-06-01
Published in Cell Biology International. London: Academic Press Ltd Elsevier B.V., v. 29, n. 6, p. 458-471, 2005.
ISSN 1065-6995 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 458-471
Origin http://dx.doi.org/10.1016/j.cellbi.2004.11.025
Access rights Closed access
Type Article
Web of Science ID WOS:000231189200008
URI http://repositorio.unifesp.br/handle/11600/28323

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