The C-terminus of murine S100A9 inhibits spreading and phagocytic activity of adherent peritoneal cells

The C-terminus of murine S100A9 inhibits spreading and phagocytic activity of adherent peritoneal cells

Author Pagano, R. L. Google Scholar
Sampaio, S. C. Google Scholar
Juliano, L. Google Scholar
Juliano, M. A. Google Scholar
Giorgi, R. Google Scholar
Institution Butantan Inst
Universidade Federal de São Paulo (UNIFESP)
Abstract Objective and design: in the present study, the effect of a synthetic peptide (H-92-G(102)) identical to the C-terminus of murine S100A9 (mS100A9p) was investigated on adherent peritoneal cell function.Materials and methods: for in vitro assays, peritoneal cells were obtained from the abdominal cavity of mice and incubated, with the different concentrations of mS100A9p, for 1 h, and then their spreading and phagocytosis activities were evaluated. for ex-vivo assays, cells obtained from animals treated for 1 h with the peptide were submitted to the mannose-receptor phagocytosis assay. Shorter homologue peptides to the C-terminus of mS100A9p were also evaluated on in vitro phagocytosis assays of Candida albicans particles.Results: mS100A9p reduced both the spreading index and phagocytic activity, in vitro and ex-vivo, independent of the receptor evaluated. the homologue peptide corresponding to the H-92-E-97 region of mS100A9p, the zinc-binding motif, was responsible for such an effect.Conclusion: These results suggest a modulator effect of the C-terminus of S100A9 protein on the function of adherent peritoneal cells.
Keywords S100A9
adherent peritoneal cells
spreading
phagocytosis
zinc
Language English
Date 2005-05-01
Published in Inflammation Research. Basel: Birkhauser Verlag Ag, v. 54, n. 5, p. 204-210, 2005.
ISSN 1023-3830 (Sherpa/Romeo, impact factor)
Publisher Birkhauser Verlag Ag
Extent 204-210
Origin http://dx.doi.org/10.1007/s00011-005-1344-y
Access rights Closed access
Type Article
Web of Science ID WOS:000229641000003
URI http://repositorio.unifesp.br/handle/11600/28273

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