Sperm retrieval techniques in rats with suppressed spermatogenesis by experimental cryptorchidism

Sperm retrieval techniques in rats with suppressed spermatogenesis by experimental cryptorchidism

Author Rossi, L. M. Google Scholar
Pereira, LAV Google Scholar
Santis, L. de Google Scholar
Pasqualotto, F. F. Google Scholar
Iaconelli Júnior, Assumpto Autor UNIFESP Google Scholar
Ortiz, V Google Scholar
Borges Júnior, Edson Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Caxias Sul
Abstract Objective: Our aim was to assess the suppression of spermatogenesis and sperm retrieval rate after testicular sperm extraction (TESE) or testicular sperm aspiration (TESA) in adult rats with surgically induced cryptorchidism. Methods: Adult rats were submitted to TESE and TESA procedures after 15 days of induced cryptorchidism. After spermatozoa retrieval, the testicles were extracted, weighed and a morphological analysis by conventional light microscopy was done. the numbers of spermatozoa retrieved in both TESA and TESE were rated and compared. Results: Histological analysis of the testicles revealed Sertoli cell-only syndrome in 60% of the testicles, and maturation arrest in the remaining cryptorchid testicles. Significant differences were seen in the number of spermatozoa retrieved (P < 0.05) between cryptorchidic and control rats. When sperm retrieval techniques were compared, no differences were detected in the number of spermatozoa obtained (P > 0.05). Conclusions: It seems that a 15 day period of cryptorchidism is enough to induce spermatogenesis disorders. No differences were detected in the number of spermatozoa retrieved in the right or left testicles, irrespective of the testicular pole. Furthermore, and even more importantly, no differences in the retrieval rate were seen between the two techniques.
Keywords aspiration
cryptorchidism
extraction
sperm
testicle
Language English
Date 2005-02-01
Published in Human Reproduction. Oxford: Oxford Univ Press, v. 20, n. 2, p. 443-447, 2005.
ISSN 0268-1161 (Sherpa/Romeo, impact factor)
Publisher Oxford Univ Press
Extent 443-447
Origin http://dx.doi.org/10.1093/humrep/deh549
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000226611300019
URI http://repositorio.unifesp.br/handle/11600/28122

Show full item record




File

File Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account