Tyrosine phosphorylation in nitric oxide-mediated signaling events

Tyrosine phosphorylation in nitric oxide-mediated signaling events

Author Monteiro, H. P. Google Scholar
Oliveira, CJR Google Scholar
Curcio, M. F. Google Scholar
Moraes, M. S. Google Scholar
Arai, R. J. Google Scholar
Packer, L. Google Scholar
Cadenas, E. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract In this chapter, we provide an overview of nitric oxide (NO)-tyrosine phosphorylation signal transduction pathways, integrating them with the cyclic guanosine monophosphate (cGMP) and S-nitrosylation-mediated pathways that are triggered by NO. the second half of this chapter includes a description of the methods that our laboratory has used extensively to characterize the mechanisms involved in signaling events mediated by this pathway. These include assays for detecting protein tyrosine phosphorylation, tyrosine phosphorylation of the epidermal growth factor (EGF) receptor, phosphorylation of the ERK1/2 mitogen-activated protein (MAP) kinases, transfection of cells with modified forms of p21Ras, and an assay of p21Ras.
Language English
Date 2005-01-01
Published in Nitric Oxide, Pt E. San Diego: Elsevier Academic Press Inc, v. 396, p. 350-358, 2005.
ISSN 0076-6879 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 350-358
Origin http://dx.doi.org/10.1016/S0076-6879(05)96029-1
Access rights Closed access
Type Review
Web of Science ID WOS:000234454400029
URI http://repositorio.unifesp.br/handle/11600/28093

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