Attaching and effacing Escherichia coli isolated from dogs in Brazil: characteristics and serotypic relationship to human enteropathogenic E-coli (EPEC)

Attaching and effacing Escherichia coli isolated from dogs in Brazil: characteristics and serotypic relationship to human enteropathogenic E-coli (EPEC)

Author Nakazato, G. Google Scholar
Gyles, C. Google Scholar
Ziebell, K. Google Scholar
Keller, R. Google Scholar
Trabulsi, L. R. Google Scholar
Gomes, TAT Google Scholar
Irino, K. Google Scholar
Da Silveira, W. D. Google Scholar
De Castro, AFP Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Estadual de Campinas (UNICAMP)
Univ Guelph
Hlth Canada
Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Dept Hlth State São Paulo
Abstract Escherichia coli isolates recovered from 182 fecal specimens from dogs up to five months old from the cities of São Paulo and Campinas, SP, Brazil, were examined by polymerase chain reaction (PCR) for several virulence factors and properties. the eae gene was found in 23 isolates of E. coli from 22 dogs, 19 of 146 (13%) from dogs with diarrhea and 3 of 36 (8.3%) from dogs with no diarrhea. Two different eae+ isolates were recovered from one dog with diarrhea. Isolates from two dogs with diarrhea harbored the bfpA gene, and none of the isolates possessed genes for enterotoxins, the EAF plasmid or Shiga toxins. PCR showed that, among the 23 isolates, eight were positive for beta intimin, six for gamma, two for epsilon, one for alpha, one for K, and five showed no amplification with any of the nine pairs of specific intimin primers used. PCR also showed that the LEE (locus of enterocyte effacement) was inserted in selC in four isolates, likely in pheU in seven isolates, and in undetermined sites in twelve isolates. Fifteen isolates adhered to HEp-2 cells and were fluorescence actin staining (FAS) positive. the predominant adherence pattern was the localized adherence-like (LAL) pattern. the eae-positive isolates belonged to a wide diversity of serotypes, including O111:H25, O119:H2 and O142:H6, which are serotypes that are common among human EPEC. These results confirmed the presence of EPEC in dogs (DEPEC) with and without diarrhea. the virulence factors found in these strains were similar to those in human EPEC, leading to the possibility that EPEC may move back and forth among human and canine populations. (C) 2004 Elsevier B.V. All rights reserved.
Keywords attaching and effacing
dog
Escherichia coli
human EPEC
serotypes
Brazil
Language English
Date 2004-08-06
Published in Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 101, n. 4, p. 269-277, 2004.
ISSN 0378-1135 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 269-277
Origin http://dx.doi.org/10.1016/j.vetmic.2004.04.009
Access rights Closed access
Type Article
Web of Science ID WOS:000223111600006
URI http://repositorio.unifesp.br/handle/11600/27885

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