Effects of androgen manipulation on alpha(1)-adrenoceptor subtypes in the rat seminal vesicle

Effects of androgen manipulation on alpha(1)-adrenoceptor subtypes in the rat seminal vesicle

Author Mendes, F. R. Google Scholar
Hamamura, M. Google Scholar
Queiroz, DBC Google Scholar
Porto, C. S. Google Scholar
Avellar, MCW Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract This study analyses possible changes during surgical and chemical castration in the expression and pharmacological characteristics of alpha(1)-adrenoceptor in adult rat seminal vesicle. Ribonuclease protection assays indicated that alpha(1a)- was the predominant mRNA, while alpha(1b)- and alpha(1d)-adrenoceptor transcripts were detected in lower abundance in this tissue. alpha(1a)-adrenoceptor mRNA expression presented a complex dependency on androgens, while alpha(1b)- and alpha(1d)-adrenoceptor transcripts were both upregulated with surgical and chemical castration, suggesting a negative modulation by androgens. Testosterone treatment reversed the effects caused by surgical castration. Functional studies confirmed the involvement of alpha(1A)- and alpha(1B)-adrenoceptor in the seminal vesicle contractile responses, and suggested that alpha(1B)-induced contractile response was upregulated after castration. Taken together, the results suggest that alpha(1)-adrenoceptor expression in seminal vesicle is differentially regulated by the androgen status of the rat. (C) 2004 Elsevier Inc. All rights reserved.
Keywords seminal vesicle
alpha(1)-adrenoceptor
castration
androgen
Language English
Date 2004-08-06
Published in Life Sciences. Oxford: Pergamon-Elsevier B.V., v. 75, n. 12, p. 1449-1463, 2004.
ISSN 0024-3205 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 1449-1463
Origin http://dx.doi.org/10.1016/j.lfs.2004.03.011
Access rights Closed access
Type Article
Web of Science ID WOS:000222873600004
URI http://repositorio.unifesp.br/handle/11600/27884

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