Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties

Effects of 'casoparan', a peptide isolated from casein hydrolysates with mastoparan-like properties

Author Lebrun, I Google Scholar
Cavallaro, V Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Silva, MCCDE Google Scholar
Institution Butantan Inst
Universidade Federal de São Paulo (UNIFESP)
Abstract CASEIN, a protein found in milk of several species, is divided into different chains from 19 to 25 kappaDa. Casein is also considered as a source of amino acids and generating peptides with biological activities such as opiate, immunostimulating, antibacterial, peptidase inhibitors, among others. in this work, Sephadex G-10 chromatography followed by high-performance liquid chromatography isolation purified NZCase TT, an industrial culture media for tetanus toxin production. in the first step, four pools were isolated and tested in different assays: isolated smooth muscle assay ( guinea pig ileum, rat uterus), phagocytosis in vitro of opsonized sheep red blood cells, and hydrogen peroxide ( H2O2) release from mouse peritoneal macrophages. Pool III was the main active pool being able to potentiate bradykinin action in guinea pig ileum, stimulating phagocitic activity by resident macrophages and increasing H2O2 release from macrophages previously activated with bacille Calmette Guerin. Using mass spectra the primary structure of the main peptide from pool III was obtained-INKKI, which corresponds to beta-casein fragment 26-30. the immunostimulating action is probably related to a direct action in macrophage cells.
Keywords mastoparan
casein peptides
peptide isolation
Language English
Date 2004-08-01
Published in Mediators of Inflammation. Basingstoke: Carfax Publishing, v. 13, n. 4, p. 263-268, 2004.
ISSN 0962-9351 (Sherpa/Romeo, impact factor)
Publisher Carfax Publishing
Extent 263-268
Origin http://dx.doi.org/10.1080/09629350400003068
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000224975100005
URI http://repositorio.unifesp.br/handle/11600/27841

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