B and T cell responses elicited by monoclonal anti-idiotypic antibody (Ab2 beta) mimicking gp43 from Paracoccidioides brasiliensis

B and T cell responses elicited by monoclonal anti-idiotypic antibody (Ab2 beta) mimicking gp43 from Paracoccidioides brasiliensis

Author Souza, E. B. Google Scholar
Lopes, Jose Daniel Autor UNIFESP Google Scholar
Almeida, SR Google Scholar
Institution Universidade de São Paulo (USP)
Universidade Federal de São Paulo (UNIFESP)
Abstract Paracoccidioidomycosis is a systemic mycosis endemic in Latin America, with a high prevalence in Brazil, Argentina, Colombia and Venezuela. the aetiological agent of disease is the thermal dimorphic fungus, Paracoccidioides brasiliensis. A glycoprotein of 43 kD (gp43) is the major antigen of P. brasiliensis. Antibodies directed to this antigen are detected in the sera of all patients with paracoccidioidomycosis (PCM). Recently, it has been shown that mice immunized with anti-gp43 monoclonal antibodies (MAbs) (Ab1), induce the idiotypic cascade in the gp43 system, which produced both, anti-Id antibodies (Ab2) and anti-anti-Id antibodies (Ab3). To further characterize the idiotypic cascade modulation in mice immunized with anti-gp43 MAb 17c, hybridomas were produced. Ab2 MAbs named 7.B12 inhibited (>95%) the binding of gp43 to MAb 17c (Ab1), suggesting that this anti-Id MAb bind to the idiotope, thus fulfilling the internal image criteria. To elucidate whether Ab2 MAb could act as antigen in serological assays, instead of gp43, sera from PCM patients were tested. Using an ELISA test, it was observed that antibodies from patients and not normal serum bound to Ab2. However, the ELISA test using Ab2 bound to the solid phase made possible to serologically monitor the patients after antifungal therapy, showing an equivalent curve when compared with ELISA test employing purified gp43. Our results also showed that, when mice were immunized with Ab2beta and their cells were exposed to gp43 in vitro, a T cell proliferation response was observed.
Keywords Paracoccidioides brasiliensis
gp43
idiotypic network
monoclonal antibodies
Language English
Date 2004-07-01
Published in Clinical and Experimental Immunology. Oxford: Blackwell Publishing Ltd, v. 137, n. 1, p. 123-128, 2004.
ISSN 0009-9104 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing Ltd
Extent 123-128
Origin http://dx.doi.org/10.1111/j.1365-2249.2004.02507.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000222050100017
URI http://repositorio.unifesp.br/handle/11600/27804

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