Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method

Detection of gp43 of Paracoccidioides brasiliensis by the loop-mediated isothermal amplification (LAMP) method

Author Endo, S. Google Scholar
Komori, T. Google Scholar
Ricci, Giannina Autor UNIFESP Google Scholar
Sano, A. Google Scholar
Yokoyama, K. Google Scholar
Ohori, A. Google Scholar
Kamei, K. Google Scholar
Franco, Marcello Fabiano de Autor UNIFESP Google Scholar
Miyaji, M. Google Scholar
Nishimura, K. Google Scholar
Institution Chiba Univ
Fujisawa Pharmaceut Co Ltd
Universidade Federal de São Paulo (UNIFESP)
Abstract Paracoccichoidomycosis is a deep mycosis Caused by the thermo-dependent dimorphic fungus Paracoccidioides brasiliensis and is prevalent in Latin American countries. We detected the species specific gp43 gene of P. brasiliensis by loop-mediated isothermal amplification (LAMP) in 22 clinical and seven armadillo-derived isolates. the amplified DNA appeared as a ladder with a specific banding pattern. the advantage of the LAMP method is speed; only 3 h were necessary for identification of the organism and diagnosis of the disease. We were also able to obtain positive results from DNA extracted from a paraffin-embedded tissue sample of paracoccidioidomycosis, suggesting that this method may achieve clinical application in the near future. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.
Keywords gp43 gene
Paracoccidioides brasiliensis
paracoccidioidomycosis
Language English
Date 2004-05-01
Published in Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 234, n. 1, p. 93-97, 2004.
ISSN 0378-1097 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 93-97
Origin http://dx.doi.org/10.1016/j.femsle.2004.03.015
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000221218300013
URI http://repositorio.unifesp.br/handle/11600/27715

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