Carboxydipeptidase activities of recombinant cysteine peptidases - Cruzain of Trypanosoma cruzi and CPB of Leishmania mexicana

Carboxydipeptidase activities of recombinant cysteine peptidases - Cruzain of Trypanosoma cruzi and CPB of Leishmania mexicana

Author Judice, Wagner AS Google Scholar
Puzer, Luciano Google Scholar
Cotrin, Simone Silva Autor UNIFESP Google Scholar
Carmona, Adriana Karaoglanovic Autor UNIFESP Google Scholar
Coombs, Graham H. Google Scholar
Juliano, Luiz Autor UNIFESP Google Scholar
Juliano, Maria Aparecida Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Univ Glasgow
Abstract The recombinant cysteine peptidases, cruzain from Trypanosoma cruzi and CPB2.8DeltaCTE from Leishmania mexicana, are cathepsin L-like and characteristically endopeptidases. in this study, we characterized the carboxydipeptidase activities of these enzymes and compared them with those of human recombinant cathepsin B and cathepsin L. the analysis used the internally quenched fluorescent peptide Abz-FRFK*-OH and some of its analogues, where Abz is ortho-aminobenzoic acid and K* is (2,4-dinitrophenyl)-epsilon-NH2-lysine. These peptides were demonstrated to be very sensitive substrates, due to the strong quenching effect of K* on the fluorescence of the Abz group. the carboxydipeptidase activity of cruzain was shown to be very similar to that of cathepsin B, while that of CPB2.8DeltaCTE is closer to the carboxydipeptidase activity of cathepsin L. the S-2 subsite architecture of cruzain and the nature of the amino acid at the P-2 position of the substrates determine its carboxydipeptidase activity and gives further and direct support to the notion that the carboxydipeptidase activity of the papain family cysteine peptidases rely on the S-2-P-2 interaction [Nagler D. K., Tam, W., Storer, A.C., Krupa, J.C., Mort, J.S. & Menard, R. (1999) Biochemistry38, 4868-4874]. Cruzain and CPB2.8DeltaCTE presented a broad pH-range for both the endo- and exo-peptidase activities, although the later is approximately one order of magnitude lower. This feature, that is not common in related mammalian cysteine peptidases, is consistent with the enzymes being exposed to different environmental conditions and having different locations during parasite development.
Keywords cathepsin B
cathepsin L
cruzipain
exoproteases
proteases
Language English
Date 2004-03-01
Published in European Journal of Biochemistry. Oxford: Blackwell Publishing Ltd, v. 271, n. 5, p. 1046-1053, 2004.
ISSN 0014-2956 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing Ltd
Extent 1046-1053
Origin http://dx.doi.org/10.1111/j.1432-1033.2004.04008.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000189052600016
URI http://repositorio.unifesp.br/handle/11600/27639

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