The largest subunit of the RNA polymerase II of Trypanosoma cruzi lacks the repeats in the carboxy-terminal domain and is encoded by several genes

The largest subunit of the RNA polymerase II of Trypanosoma cruzi lacks the repeats in the carboxy-terminal domain and is encoded by several genes

Author Ejchel, Tatiana Flank Autor UNIFESP Google Scholar
Ramirez, M. I. Google Scholar
Vargas, N. Google Scholar
Azevedo, E. B. Google Scholar
Elias, M. C. Google Scholar
Zingales, B. Google Scholar
Schenkman, S. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Universidade de São Paulo (USP)
Abstract The largest subunit of eukaryotic RNA polymerase II (RNA Pol II) of several members of Kinetoplastida order and other early diverging eukaryote contains a serine, tyrosine and proline-rich domain in the carboxyl-terminal instead of the typical heptapeptides repeats found in most eukaryotes. the lack of these repeats seems to reflect the different control of gene transcription found in these organisms. To provide further understanding in these mechanisms, we have characterized the largest subunit of RNA polymerase II (RNA Pol II LSU) in Trypanosoma cruzi. We found that it also lacks the heptapeptide repeats in the carboxy-terminal domain, but is quite similar to, the same region in the Trypanosoma brucei enzyme, suggesting a conserved role for this domain. in addition, we found several genes encoding the RNA Pol II in T cruzi, distributed in different chromosomal bands in several isolates. Two of these genes were entirely sequenced and shown to be quite similar. the presence of several gene copies of the RNA Pol II LSU, also described in African trypanosomes might reflect a gene amplification requirement that appeared early in the evolution of these organisms. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.
Keywords Trypanosoma cruzi
RNA polymerase II
gene
transcription
karyotype
Language English
Date 2003-09-01
Published in Parasitology International. Clare: Elsevier Sci Ireland Ltd, v. 52, n. 3, p. 243-249, 2003.
ISSN 1383-5769 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 243-249
Origin http://dx.doi.org/10.1016/S1383-5769(03)00009-6
Access rights Closed access
Type Article
Web of Science ID WOS:000185972000007
URI http://repositorio.unifesp.br/handle/11600/27398

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