HSP70 induced by hyperosmotic stress partially protects LLC-PK1 cells against nephrotoxic drugs

HSP70 induced by hyperosmotic stress partially protects LLC-PK1 cells against nephrotoxic drugs

Author Leitao, A. A. Google Scholar
Leite, C. A. Google Scholar
Santos, B. C. Google Scholar
Boim, M. A. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Accumulation of HSP70 is related to the cytoprotection. It was evaluated whether hyperosmotic stress induces HSP70 accumulation in LLC-PK1 cells, and protects cells against toxicity provoked by cisplatin (Cis) and cyclosporine A (CyA). Cells were maintained in isosmotic (Iso) or hyperosmotic (H) culture medium for 24 h and then exposed to Cis or CyA for an additional period of 12 or 24 h (groups H+Cis and H+CyA). the H medium did not induce cell death and increased both HSP70 mRNA and protein levels, suggesting a role in cell adaptation to H condition. H medium produced partial cytoprotection against Cis and CyA compared with control cells. Despite the cytoprotection, there was a reduction in HSP70 mRNA and protein levels in H+Cis group. in contrast, the H+CyA group presented high levels of HSP70 mRNA and protein. the induction of HSP70 by H medium was associated with tolerance of LLC-PK1 cells against Cis and CyA cytotoxicity but this protection was induced by different mechanisms and depended on the characteristics of the drug used. Copyright (C) 2003 S. Karger AG, Basel.
Keywords hyperosmotic stress
cisplatin
cyclosporine
LLC-PK1 cells
Language English
Date 2003-07-01
Published in Nephron Experimental Nephrology. Basel: Karger, v. 94, n. 3, p. E94-E102, 2003.
ISSN 1660-2129 (Sherpa/Romeo, impact factor)
Publisher Karger
Extent E94-E102
Origin http://dx.doi.org/10.1159/000072027
Access rights Closed access
Type Article
Web of Science ID WOS:000186538000003
URI http://repositorio.unifesp.br/handle/11600/27319

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