Serotypes and Shiga toxin genotypes among Escherichia coli isolated from animals and food in Argentina and Brazil

Serotypes and Shiga toxin genotypes among Escherichia coli isolated from animals and food in Argentina and Brazil

Author Guth, BEC Google Scholar
Chinen, I Google Scholar
Miliwebsky, E. Google Scholar
Cerqueira, AMF Google Scholar
Chillemi, G. Google Scholar
Andrade, JRC Google Scholar
Baschkier, A. Google Scholar
Rivas, M. Google Scholar
Institution ANLIS Dr Carlos G Malbran
Universidade Federal de São Paulo (UNIFESP)
Universidade Federal Fluminense (UFF)
Universidade do Estado do Rio de Janeiro (UERJ)
Abstract Shiga toxin (Stx)-producing Escherichia coli (STEC) strains isolated from animals and food in Argentina (n = 44) and Brazil (n = 20) were examined and compared in regard to their phenotypic and genotypic characteristics to evaluate their pathogenic potential. the clonal relatedness of STEC O157 isolates (n = 22) was established by phage typing (PT) and pulsed-field gel electrophoresis (PFGE). All O157 strains studied carried eae and enterohemorrhagic E. coli (EHEC)-hly sequences. in Argentina, these strains occurred both in cattle and meat, and 50% of them carried stx2/stx2vh-a genes, whereas in Brazil the O157 strains were isolated from animals, and most harbored the stx2vh-a sequence. At least 13 different O:H serotypes were identified among the non-O157 strains studied, with serotype O113:H21 being found in both countries. All but one non-O157 strains did not carry eae gene, but EHEC-hlyA gene was found in 85.7% of them, and the stx2 genotype was also more prevalent in Argentina than in Brazil (P < 0.01), where stx1 alone or in association was most common (68.8%). One STEC strain isolated from a calf in Brazil harbored the new variant referred to as stx2-NV206. PFGE analysis showed that STEC O157 strains were grouped in four clusters. One Brazilian strain was considered possibly related (>= 80%) to Argentinean strains of cluster I. Differences in the pathogenic potential, especially in regard to serotypes and stx genotypes, were observed among the STEC strains recovered from animals and food in both countries. (C) 2002 Elsevier Science B.V. All rights reserved.
Keywords STEC
Language English
Date 2003-04-29
Published in Veterinary Microbiology. Amsterdam: Elsevier B.V., v. 92, n. 4, p. 335-349, 2003.
ISSN 0378-1135 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 335-349
Access rights Closed access
Type Article
Web of Science ID WOS:000181156800004

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