Combined TUNEL and TRAP methods suggest that apoptotic bone cells are inside vacuoles of alveolar bone osteoclasts in young rats

Combined TUNEL and TRAP methods suggest that apoptotic bone cells are inside vacuoles of alveolar bone osteoclasts in young rats

Author Cerri, P. S. Google Scholar
Boabaid, F. Google Scholar
Katchburian, E. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
São Paulo State Univ
Abstract Although it is generally accepted that osteoclasts breakdown and resorb bone matrix, the possibility that they may also be able to engulf apoptotic osteoblasts/ lining cells and/or osteocytes remains controversial. Apoptosis of osteoblasts/ lining cells and/or osteocytes and interactions between these cells and osteoclasts are extremely rapid events that are difficult to observe in viva. A suitable in viva model for studying these events is the alveolar bone of young rats because it is continuously. Thus, sections of aldehyde fixed alveolar undergoing intense resorption/remodeling bone of young rats were stained by the combined terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) method and the tartrate-resistant acid phosphatase (TRAP) method for the simultaneous visualization of apoptotic cells and osteoclasts in the same section. the combined TUNEL and TRAP reactions, in the same section, greatly facilitated visualization of relationship between osteoclasts and apoptotic bone cells during alveolar bone remodeling. Our results showed that several TRAP-positive osteoclasts exhibited large vacuoles containing TUNEL positive apoptotic structures, probably derived from osteoblasts/lining cells and/or osteocytes. These results support the idea that alveolar bone osteoclasts are able to internalize dying apoptotic bone cells.
Keywords alveolar bone
apoptosis
osteoclasts
bone resorption
Language English
Date 2003-04-01
Published in Journal of Periodontal Research. Copenhagen: Blackwell Munksgaard, v. 38, n. 2, p. 223-226, 2003.
ISSN 0022-3484 (Sherpa/Romeo, impact factor)
Publisher Blackwell Munksgaard
Extent 223-226
Origin http://dx.doi.org/10.1034/j.1600-0765.2003.02006.x
Access rights Closed access
Type Article
Web of Science ID WOS:000181797500017
URI http://repositorio.unifesp.br/handle/11600/27196

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