GM1 ganglioside attenuates convulsions and thiobarbituric acid reactive substances production induced by the intrastriatal injection of methylmalonic acid

GM1 ganglioside attenuates convulsions and thiobarbituric acid reactive substances production induced by the intrastriatal injection of methylmalonic acid

Author Fighera, M. R. Google Scholar
Bonini, J. S. Google Scholar
Oliveira, T. G. de Google Scholar
Frussa, R. Google Scholar
Rocha, JBT Google Scholar
Dutra, C. S. Google Scholar
Rubin, M. A. Google Scholar
Mello, C. F. Google Scholar
Institution Universidade Federal de Sergipe (UFS)
Universidade Federal de São Paulo (UNIFESP)
Univ Fed Rio Grande do Sul
Abstract The effects of the administration of monosialoganglioside (GM1) on methylmalonic acid (MMA)-induced convulsions, production of thiobarbituric acid reactive substances (TBARS) and on the striatal content of ascorbic acid and total non-protein thiol (SH) groups were evaluated in adult male rats. Animals received two intraperitoneal injections of GM1 (50 mg/kg) or saline (0.85% NaCl) spaced 24 h apart. Thirty minutes after the second GM1 or saline injection, L-MMA (6 mumol) or NaCl (9 mumol) was injected into the right striatum and the animals were observed for the appearance of convulsions for 15 min. the animals were sacrificed and their striatal content of ascorbic acid, SH groups and TBARS was measured. the effect of GM1 on MMA-induced TBARS production in striatal homogenates was also evaluated in vitro.MMA injection caused convulsions (Sal-MMA: 9.8 +/- 1.4 episodes, which lasted 271 +/- 48 s) and increased the striatal content of TBARS (Sal-MMA: 149.0 +/- 11.5 nmol MDA/g tissue), but did not alter total striatal SH or ascorbic acid contents. GM1 pretreatment decreased MMA-induced convulsions (GM1-MMA: 6.3 +/- 2.0 episodes, which lasted 115.1 +/- 42.2 s) and TBARS increase (GM1-MMA: 102.4 +/- 19.5 nmol MDA/g tissue). GM1 pretreatment increased ascorbic acid content of the striata (saline-pretreated: 1514 +/- 75.9; GM1-pretreated: 1878.6 +/- 102.8 mug ascorbic acid/mg tissue). MMA increased TBARS production in vitro, and GM1 had no effect on such MMA-induced effect.This study provides evidence that GM1 increases striatal ascorbic acid content and decreases MMA-induced neurotoxicity assessed by behavioral and neurochemical parameters. (C) 2003 Elsevier B.V. All rights reserved.
Keywords L-methylmalonic acid
convulsion
monosialoganglioside
thiobarbituric acid reactive substances
ascorbic acid
Language English
Date 2003-04-01
Published in International Journal of Biochemistry & Cell Biology. Oxford: Pergamon-Elsevier B.V., v. 35, n. 4, p. 465-473, 2003.
ISSN 1357-2725 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 465-473
Origin http://dx.doi.org/10.1016/S1357-2725(02)00275-3
Access rights Closed access
Type Article
Web of Science ID WOS:000181383200009
URI http://repositorio.unifesp.br/handle/11600/27194

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