T cell subpopulations in myocardial inflammatory infiltrates detected by confocal microscopy: dose dependence in mice treated with cyclophosphamide during acute Trypanosoma cruzi infection

T cell subpopulations in myocardial inflammatory infiltrates detected by confocal microscopy: dose dependence in mice treated with cyclophosphamide during acute Trypanosoma cruzi infection

Author Calabrese, K. S. Google Scholar
Paradela, ASRC Google Scholar
Valle, T. Z. do Google Scholar
Tedesco, R. C. Google Scholar
Leonardo, R. Google Scholar
Mortara, R. A. Google Scholar
Costa, SCG da Google Scholar
Institution Inst Oswaldo Cruz
Universidade Federal de São Paulo (UNIFESP)
Abstract In this article, we have characterized cell subpopulations found in the hearts of mice presenting acute Chagas' disease by immunocytochemistry and subjected to different schedules of an immunosuppressive therapy with cyclophosphamide (CY). in this comparative study, CY treatment with different doses was carried out before or after infection with Trypanosoma cruzi Y strain trypomastigotes, enabling us to discriminate the parasitemic kinetics and inflammatory processes in the heart, 12 d after infection. Animals treated with 200 mg/kg of CY 2 d before infection presented high parasitaemia as well as heavy inflammation and low parasite loads in the heart. Mice treated 5 d after infection with the same dose, developed the same parasitaemic peak but were not able to control it. Their heart did not present inflammation, but a high number of parasites could be seen. Animals treated with five 3 mg/kg doses of CY every other day presented heavy inflammatory reaction and low parasitaemia. in this group, as well as the one treated before infection, immunocytochemistry studies have shown predominance of CD8(+) T cells in the myocardium. On the other hand, mice treated with 200 mg/kg of CY 5 d after infection, presented small amounts of CD4(+) T cells while no CD8(+) could be found. These results have confirmed the dose dependence influence of this drug on the T cell populations in the inflammatory infiltrates as well as the importance of the schedule employed. (C) 2003 Editions scientifiques et medicales Elsevier SAS. All rights reserved.
Keywords Chagas' disease
confocal microscopy
cyclophosphamide
immunomodulation
Language English
Date 2003-04-01
Published in Pathologie Biologie. Paris Cedex 15: Editions Scientifiques Medicales Elsevier, v. 51, n. 3, p. 129-134, 2003.
ISSN 0369-8114 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 129-134
Origin http://dx.doi.org/10.1016/S0369-8114(03)00022-1
Access rights Closed access
Type Article
Web of Science ID WOS:000184219500002
URI http://repositorio.unifesp.br/handle/11600/27192

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