Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies

Evidence that ATP participates in the pathophysiology of pilocarpine-induced temporal lobe epilepsy: Fluorometric, immunohistochemical, and Western blot studies

Author Vianna, Eduardo Paulo Morawski Autor UNIFESP Google Scholar
Ferreira, Alice Teixeira Autor UNIFESP Google Scholar
Naffah-Mazzacoratti, Maria da Graca Autor UNIFESP Google Scholar
Sanabria, Emilio Rafael Garrido Autor UNIFESP Google Scholar
Funke, M. Autor UNIFESP Google Scholar
Cavalheiro, Esper Abrão Autor UNIFESP Google Scholar
Fernandes, Maria Jose da Silva Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Purpose: This study was performed to study the role of adenosine triphosphate (ATP) in the brain of pilocarpine-induced chronic epileptic rats.Methods: ATP-mediated changes in intracellular calcium were studied by the fura-2 method. lmmunohistochemistry and Western blotting methods were used to localize and quantify P2X7 receptors in these animals.Results: the fluorometric study in chronic rats revealed a biphasic response indicating the presence of P2X7 receptors. the Western blotting study showed an increase of 80% of P2X7 expression in chronic rats compared with the control group. P2X7 immunoreactivity resembled mossy fiber sprouting at the dentate gyrus of epileptic animals.Conclusions: These results suggest that purinergic receptors may participate in the pathophysiology of temporal lobe epilepsy.
Keywords epilepsy
ATP
P2X7
pilocarpine
rat
Language English
Date 2002-01-01
Published in Epilepsia. Malden: Blackwell Publishing Inc, v. 43, p. 227-229, 2002.
ISSN 0013-9580 (Sherpa/Romeo, impact factor)
Publisher Blackwell Publishing Inc
Extent 227-229
Origin http://dx.doi.org/10.1046/j.1528-1157.43.s.5.26.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000177445900042
URI http://repositorio.unifesp.br/handle/11600/26681

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