Autoantibodies specificity in acute anterior uveitis according to the presence of the HLA-B27 allele

Autoantibodies specificity in acute anterior uveitis according to the presence of the HLA-B27 allele

Author Trevisani, VFM Google Scholar
Mattos, KTF Google Scholar
Esteves, R. F. Google Scholar
Olialves, SMR Google Scholar
Andrade, LEC Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Purpose: To study HLA-B27circle plus and HLA-B27O patients with AAU with respect to the frequency and specificity of organ-specific and non-organ-specific autoantibodies. Methods: Fifty-seven consecutive patients with AAU were subjected to ophthalmologic and rheumatologic evaluation as well as to autoantibody determination: antinuclear antibodies (ANA), rheumatoid factor (RF), anticardiolipin, anti-smooth muscle, and anti-parietal cell antibodies, and immunoblot for antibodies to HeLa cells and to bovine iris extract. HLA-B27 was determined by a microlymphocytotoxicity assay. Statistical analysis employed chi-square test, Fisher's exact test, and McNemar test. Results: Thirty-four patients had the HLA-B27 allele (B27circle plus/AAU) and 23 did not (B27O/AAU). ANA, RF, anticardiolipin, and anti-parietal cell antibodies appeared in low frequency. No patient presented anti-smooth muscle antibody. Immunoblot showed a high frequency of antibodies to HeLa cell proteins in B27O/AAU patients with predominant reactivity at 46 kDa and 56kDa. in contrast, sera from B27circle plus/AAU patients reacted poorly against HeLa cell antigens. Immunoblot with bovine iris extract showed a significant frequency of antibodies in both groups, with a predominant response to antigens with an estimated mobility of 35, 52, and 54 kDa. Conclusion: Antibodies specific to iris antigens were equally frequent in both acute uveitis groups, whereas non-organ-specific autoantibodies, especially those to HeLa cell proteins, were far less frequent in B27circle plus/AAU than in B27O/AAU patients.
Keywords autoantibodies
anterior uveitis
Language English
Date 2001-01-01
Published in Ocular Immunology and Inflammation. Lisse: Swets Zeitlinger Publishers, v. 9, n. 4, p. 231-242, 2001.
ISSN 0927-3948 (Sherpa/Romeo, impact factor)
Publisher Swets Zeitlinger Publishers
Extent 231-242
Access rights Closed access
Type Article
Web of Science ID WOS:000174654800002

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