Prevalence of HEp-2 cell-adherent Escherichia coli and characterisation of enteroaggregative E-coli and chain-like adherent E-coli isolated from children with and without diarrhoea, in Londrina, Brazil

Prevalence of HEp-2 cell-adherent Escherichia coli and characterisation of enteroaggregative E-coli and chain-like adherent E-coli isolated from children with and without diarrhoea, in Londrina, Brazil

Author Gioppo, Nereida MR Google Scholar
Elias Junior, Waldir Pereira Autor UNIFESP Google Scholar
Vidotto, Marilda C. Google Scholar
Linhares, Rosa E. Google Scholar
Saridakis, Halha O. Google Scholar
Gomes, Tania Aparecida Tardelli Autor UNIFESP Google Scholar
Trabulsi, Luiz R. Google Scholar
Pelayo, Jacinta S. Google Scholar
Institution Universidade Estadual de Londrina (UEL)
Inst Butantan
Universidade Federal de São Paulo (UNIFESP)
Abstract A total of 919 Escherichia coli isolates from 125 children with diarrhoea (cases) and 98 controls were assayed for adherence to HEp-2 cells. Localised adherence was found only in isolates from cases. Diffuse, aggregative (AA), chain-like adherence (CLA) and variants of the AA pattern were found in both cases and controls. the AA isolates were tested for gene sequences associated with enteroaggregative E. coli (EAEC). Only 25% of the isolates hybridised with the EAEC probe, and the aafA, astA and pet gene sequences were found in 7.9%, 44.7% and 7.9% of the isolates, respectively. the aggA gene was not found, although 7.9% were positive for aggC. the CLA isolates reacted with the EAEC probe (55.6%),nd the aggC, astA and pet gene sequences were found in 66.7%, 33.3% and 11.1%, respectively. the aggR (55.6%), aspU (55.6%), shf(33.3%) and she (22.2%) genes were also found in CLA isolates. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Keywords adherence pattern
enteroaggregative Escherichia coli
chain-like adhesion
virulence marker
Language English
Date 2000-09-15
Published in Fems Microbiology Letters. Amsterdam: Elsevier B.V., v. 190, n. 2, p. 293-298, 2000.
ISSN 0378-1097 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 293-298
Origin http://dx.doi.org/10.1111/j.1574-6968.2000.tb09301.x
Access rights Open access Open Access
Type Article
Web of Science ID WOS:000089209700019
URI http://repositorio.unifesp.br/handle/11600/26384

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