CD4 Th1 but not Th2 clones efficiently activate macrophages to eliminate Trypanosoma cruzi through a nitric oxide dependent mechanism

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dc.contributor.author Rodrigues, M. M.
dc.contributor.author Ribeirao, M.
dc.contributor.author Boscardin, S. B.
dc.date.accessioned 2016-01-24T12:31:07Z
dc.date.available 2016-01-24T12:31:07Z
dc.date.issued 2000-07-03
dc.identifier http://dx.doi.org/10.1016/S0165-2478(00)00205-4
dc.identifier.citation Immunology Letters. Amsterdam: Elsevier B.V., v. 73, n. 1, p. 43-50, 2000.
dc.identifier.issn 0165-2478
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26343
dc.description.abstract We have recently generated CD4 clones from BALB/c mice immunized with a plasmid DNA containing the gene encoding for the catalytic domain of trans-sialidase, an important enzyme expressed on the surface of Trypanosoma cruzi trypomastigotes. These clones allowed us to study in vitro the interaction between T cells and T. cruzi-infected macrophages. A cytotoxic CD4 clone of the Th1 type effectively activated macrophages to kill intracellular amastigote forms of T. cruzi. in contrast, CD4 Th2-like clones were much less efficient, being unable to activate macrophages to significantly reduce parasite development. We found that the anti-parasitic activity of Th1 cells was completely suppressed by the presence of nitric oxide synthase inhibitors. Also, we observed that anti-IFN-gamma antibodies significantly inhibited the anti-parasitic activity of these cells. We conclude that trypomastigote-specific Th1 cells activate macrophages to kill intracellular amastigotes of T. cruzi by a mechanism exclusively dependent on the induction of nitric oxide synthesis. (C) 2000 Elsevier Science B.V. All rights reserved. en
dc.format.extent 43-50
dc.language.iso eng
dc.publisher Elsevier B.V.
dc.relation.ispartof Immunology Letters
dc.rights Acesso restrito
dc.subject Trypanosoma cruzi en
dc.subject CD4 Th1 clone en
dc.subject nitric oxide en
dc.title CD4 Th1 but not Th2 clones efficiently activate macrophages to eliminate Trypanosoma cruzi through a nitric oxide dependent mechanism en
dc.type Artigo
dc.rights.license http://www.elsevier.com/about/open-access/open-access-policies/article-posting-policy
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.description.affiliation Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Escola Paulista Med, Dept Microbiol Imunol & Parasitol, BR-04023062 São Paulo, Brazil
dc.identifier.doi 10.1016/S0165-2478(00)00205-4
dc.description.source Web of Science
dc.identifier.wos WOS:000088604900008



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