CD4 Th1 but not Th2 clones efficiently activate macrophages to eliminate Trypanosoma cruzi through a nitric oxide dependent mechanism

CD4 Th1 but not Th2 clones efficiently activate macrophages to eliminate Trypanosoma cruzi through a nitric oxide dependent mechanism

Author Rodrigues, M. M. Google Scholar
Ribeirao, M. Google Scholar
Boscardin, S. B. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We have recently generated CD4 clones from BALB/c mice immunized with a plasmid DNA containing the gene encoding for the catalytic domain of trans-sialidase, an important enzyme expressed on the surface of Trypanosoma cruzi trypomastigotes. These clones allowed us to study in vitro the interaction between T cells and T. cruzi-infected macrophages. A cytotoxic CD4 clone of the Th1 type effectively activated macrophages to kill intracellular amastigote forms of T. cruzi. in contrast, CD4 Th2-like clones were much less efficient, being unable to activate macrophages to significantly reduce parasite development. We found that the anti-parasitic activity of Th1 cells was completely suppressed by the presence of nitric oxide synthase inhibitors. Also, we observed that anti-IFN-gamma antibodies significantly inhibited the anti-parasitic activity of these cells. We conclude that trypomastigote-specific Th1 cells activate macrophages to kill intracellular amastigotes of T. cruzi by a mechanism exclusively dependent on the induction of nitric oxide synthesis. (C) 2000 Elsevier Science B.V. All rights reserved.
Keywords Trypanosoma cruzi
CD4 Th1 clone
nitric oxide
Language English
Date 2000-07-03
Published in Immunology Letters. Amsterdam: Elsevier B.V., v. 73, n. 1, p. 43-50, 2000.
ISSN 0165-2478 (Sherpa/Romeo, impact factor)
Publisher Elsevier B.V.
Extent 43-50
Origin http://dx.doi.org/10.1016/S0165-2478(00)00205-4
Access rights Closed access
Type Article
Web of Science ID WOS:000088604900008
URI http://repositorio.unifesp.br/handle/11600/26343

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