Content of liver and brain ubiquinol-9 and ubiquinol-10 after chronic ethanol intake in rats subjected to two levels of dietary alpha-tocopherol

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dc.contributor.author Junqueira, VBC
dc.contributor.author Carrasquedo, F.
dc.contributor.author Azzalis, L. A.
dc.contributor.author Giavarotti, KAS
dc.contributor.author Giavarotti, L.
dc.contributor.author Rodrigues, L.
dc.contributor.author Fraga, C. G.
dc.contributor.author Boveris, A.
dc.contributor.author Videla, L. A.
dc.date.accessioned 2016-01-24T12:30:59Z
dc.date.available 2016-01-24T12:30:59Z
dc.date.issued 2000-01-01
dc.identifier http://dx.doi.org/10.1080/10715760000301481
dc.identifier.citation Free Radical Research. Reading: Harwood Acad Publ Gmbh, v. 33, n. 3, p. 313-319, 2000.
dc.identifier.issn 1071-5762
dc.identifier.uri http://repositorio.unifesp.br/handle/11600/26228
dc.description.abstract To assess the effect of chronic ethanol ingestion in the content of the reduced forms of coenzymes Q(9) (ubiquinol-9) and Q(10) (ubiquinol-10) as a factor contributing to oxidative stress in liver and brain, male Wistar rats were fed ad libitum a basal diet containing either 10 or 2.5 mg alpha-tocopherol/100% diet (controls), or the same basal diet plus a 32% ethanol-25% sucrose solution. After three months treatment, ethanol chronically-treated rats showed identical growth rates to the isocalorically pair-fed controls, irrespectively of alpha-tocopherol dietary level. Lowering dietary alpha-tocopherol led to a decreased content of this vitamin in the liver and brain of control rats, without changes in that of ubiquinol-9, and increased levels of hepatic ubiquinol-10 and total glutathione (tGSH), accompanied by a decrease in brain tGSH. At the two levels of dietary alpha-tocopherol, ethanol treatment significantly decreased the content of hepatic alpha-tocopherol and ubiquinols 9 and 10. This effect was significantly greater at 10 mg alpha-tocopherol/100% diet than at 2.5, whereas those of tGSH were significantly elevated by 43% and 9%, respectively. Chronic ethanol intake did not alter the content of brain alpha-tocopherol and tGSH, whereas those of ubiquinol-9 were significantly lowered by 20% and 14% in rats subjected to 10 and 2.5 mg alpha-tocopherol/100 g diet, respectively. It is concluded that chronic ethanol intake at two levels of dietary alpha-tocopherol induces a depletion of hepatic alpha-tocopherol and ubiquinols 9 and 10, thus contributing to ethanol-induced oxidative stress in the liver tissue. This effect of ethanol is dependent upon the dietary level of alpha-tocopherol, involves a compensatory enhancement in hepatic tGSH availability, and is not observed in the brain tissue, probably due to its limited capacity for ethanol biotransformation and glutathione synthesis. en
dc.format.extent 313-319
dc.language.iso eng
dc.publisher Harwood Acad Publ Gmbh
dc.relation.ispartof Free Radical Research
dc.rights Acesso restrito
dc.subject chronic ethanol ingestion en
dc.subject ubiquinols en
dc.subject alpha-tocopherol en
dc.subject total glutathione en
dc.subject liver en
dc.subject brain en
dc.title Content of liver and brain ubiquinol-9 and ubiquinol-10 after chronic ethanol intake in rats subjected to two levels of dietary alpha-tocopherol en
dc.type Artigo
dc.contributor.institution Universidade Federal de São Paulo (UNIFESP)
dc.contributor.institution Univ Buenos Aires
dc.contributor.institution Universidade de São Paulo (USP)
dc.contributor.institution Univ Chile
dc.description.affiliation Universidade Federal de São Paulo, Dept Med, Disciplina Geriatr, BR-04039032 São Paulo, Brazil
dc.description.affiliation Univ Buenos Aires, Fac Farm & Bioquim, RA-1113 Buenos Aires, DF, Argentina
dc.description.affiliation Univ São Paulo, Inst Quim, Dept Bioquim, BR-01498 São Paulo, Brazil
dc.description.affiliation Univ Chile, Fac Med, Inst Ciencias Biomed, Programa Farmacol Mol & Clin, Santiago 7, Chile
dc.description.affiliationUnifesp Universidade Federal de São Paulo, Dept Med, Disciplina Geriatr, BR-04039032 São Paulo, Brazil
dc.identifier.doi 10.1080/10715760000301481
dc.description.source Web of Science
dc.identifier.wos WOS:000089221500011



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