Comparative study of agar diffusion test and the NCCLS macrobroth method for in vitro susceptibility testing of Candida spp.

Comparative study of agar diffusion test and the NCCLS macrobroth method for in vitro susceptibility testing of Candida spp.

Author Soni, LMF Google Scholar
Burattini, M. N. Google Scholar
Pignatari, ACC Google Scholar
Gompertz, O. F. Google Scholar
Colombo, A. L. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract We performed a prospective double-blind study to evaluate the correlation between inhibition zones obtained by a disk-diffusion test, using Neo-sensitabs of fluconazole (Rosco Diagnostica), and the MICs generated by the NCCLS macrobroth dilution assay. Eighty clinical isolates, representing 5 of the clinically relevant species of Candida, were tested simultaneously by both methods. A clear inverse correlation was found between the results obtained by both tests (r = -0.69). in addition, there was high degree of agreement between methods in the identification of susceptible isolates. However, the resistance definition by disk-diffusion test had a positive predictive value of only 17%. Our data support the hypothesis that Rosco Fluconazole Neo-sensitabs have potential as a screening test for the identification of Candida isolates susceptible to fluconazole. Resistant isolates should be further investigated by standardized broth procedures.
Keywords Candida
resistance
susceptibility testing
Language English
Date 1999-01-01
Published in Mycopathologia. Dordrecht: Kluwer Academic Publ, v. 145, n. 3, p. 131-135, 1999.
ISSN 0301-486X (Sherpa/Romeo, impact factor)
Publisher Kluwer Academic Publ
Extent 131-135
Origin http://dx.doi.org/10.1023/A:1007068826861
Access rights Closed access
Type Article
Web of Science ID WOS:000084487200005
URI http://repositorio.unifesp.br/handle/11600/26011

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