THE DETECTION of PHOSPHOLIPASE-RESISTANT and PHOSPHOLIPASE-SENSITIVE GLYCOSYL-PHOSPHATIDYLINOSITOL MEMBRANE ANCHORS BY WESTERN BLOTTING

THE DETECTION of PHOSPHOLIPASE-RESISTANT and PHOSPHOLIPASE-SENSITIVE GLYCOSYL-PHOSPHATIDYLINOSITOL MEMBRANE ANCHORS BY WESTERN BLOTTING

Author Guther, MLS Google Scholar
Dealmeida, MLC Google Scholar
Rosenberry, T. L. Google Scholar
Ferguson, MAJ Google Scholar
Institution CASE WESTERN RESERVE UNIV
UNIV DUNDEE
Universidade Federal de São Paulo (UNIFESP)
Abstract Glycosyl-phosphatidylinositol (GPI) membrane anchors are present on a large number of eukaryotic plasma membrane proteins. Some of these anchors can be cleaved with bacterial phosphatidylinositol-specific phospholipases C, and a glycosyl-phosphatidylinositol-specific phospholipase C from Trypanosoma brucei, to reveal an epitope called the cross-reacting determinant. Other glycosyl-phosphatidylinositol anchors are resistant to the action of these enzymes prior to treatment with mild base. A simple method is described for identifying both phospholipase-sensitive and -resistant anchors using anti-cross-reacting determinant antibodies on Western blots. This procedure represents a high-sensitivity general method for the identification of GPI-anchored proteins. (C) 1994 Academic Press, Inc.
Language English
Date 1994-06-01
Published in Analytical Biochemistry. San Diego: Academic Press Inc Jnl-comp Subscriptions, v. 219, n. 2, p. 249-255, 1994.
ISSN 0003-2697 (Sherpa/Romeo, impact factor)
Publisher Academic Press Inc Jnl-comp Subscriptions
Extent 249-255
Origin http://dx.doi.org/10.1006/abio.1994.1264
Access rights Closed access
Type Article
Web of Science ID WOS:A1994NN59000011
URI http://repositorio.unifesp.br/handle/11600/25410

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