Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi

Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi

Alternative title Caracterização antigênica de tripomastigotas metacíclicos de Trypanosoma cruzi provenientes do vetor e de cultura
Author Yoshida, Nobuko Autor UNIFESP Google Scholar
Teixeira, Marta Maria Geraldes Autor UNIFESP Google Scholar
Sbravate, Clizete Aparecida Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics. They were found to be similar by the various parameters examined. Thus, sera of mice immunized with either one of the two metacyclic types precipitated a 82Kd surface protein from 131I-labeled culture metacyclics. Sera of mice protected against acute T. cruzi infection by immunization with killed culture metacyclics of a different strain (G) recognized, by immunoblotting, a 77Kd protein in both types of CL strain metacyclics. A monoclonal antibody raised against G strain metacyclics, and specific for metacyclic stages of this strain, reacted with both CL strain metacyclic types. Both metacyclic forms were similarly Iysed by various anti-T. cruzi sera, in a complement-mediated reaction.

Tripomastigotas metacíclicos da cepa CL de Trypanosoma cruzi, originários de triatomineos e de cultura axênica, foram comparativamente analisados quanto a sua composição antigênica e características ímunogênicas. Os vários parâmetros examinados mostraram que as duas formas metacíclicas sáo semelhantes. Assim, soros de camundongos imunizados com qualquer uma das formas metacíclicas precipitaram uma proteína de superfície de 82Kd de tripomastigotas metacíclicos de cultura marcados com 131I. Soros de camundongos protegidos con trn infecção aguda por T. cruzi, por imunização com formas metacíclicas de cultura inativadas, de uma cepa diferente (G), detectaram por imunoblotting uma proteína de 77Kd em ambas as formas metacíclicas da cepa CL. Um anticorpo monoclonal, produzido contra as for mas metaciclicas da cepa G, e específico para tripomastigotas metacíclicos, reagiu com formas metacíclicas da cepa CL tanto do vetor quanto de cultura. As duas formas metacíclicas mostraram suscetibilidade semelhante à lise por vários soros anti-T. cruzi, em uma reação mediada por complemento.
Keywords Chagas'disease
vector borne
Trypanosoma cruzi, cultured metacyclic tripomastigotes
Language English
Sponsor Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
Date 1986-04-01
Published in YOSHIDA, Nobuko; TEIXEIRA, Marta Maria Geraldes; SBRAVATE, Clizete Aparecida. Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi. Rev. Inst. Med. trop. S. Paulo, São Paulo , v. 28, n. 2, p. 80-86, abr. 1986
ISSN 0036-4665 (Sherpa/Romeo, impact factor)
Publisher Instituto de Medicina Tropical
Extent 80-86
Origin http://dx.doi.org/10.1590/S0036-46651986000200003
Access rights Open access Open Access
Type Article
Web of Science ID WOS:A1986C847900003
SciELO ID S0036-46651986000200003 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/209

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