Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay

Detection of group A beta-hemolytic Streptococcus employing three different detection methods: culture, rapid antigen detecting test, and molecular assay

Author Santos, Odimara Autor UNIFESP Google Scholar
Weckx, Luc Louis Maurice Autor UNIFESP Google Scholar
Pignatari, Antonio Carlos Campos Autor UNIFESP Google Scholar
Pignatari, Shirley Shizue Nagata Autor UNIFESP Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
Abstract In order to study the prevalence of Group A beta-hemolytic Streptococcus (GABHS) pharyngotonsillitis in our pediatric population and to compare different sampling methods of GABHS detection, oropharyngeal swabs from 50 children with acute pharyngotonsillitis, between 1 and 12 years old, were used simultaneously for culture, molecular assay and rapid GABHS antigen detection tests. All children were clinically examined at the Division of Pediatric Otorhinolaryngology of the Federal University of São Paulo. Diagnostic criteria were based on signs and symptoms, including sore throat, fever and oropharyngeal purulent secretion. Children that had been treated with antibiotics were excluded. Overall, combining the three methods, the prevalence of GABHS was 34%. GABHS was diagnosed in 30% of the bacterial cultures, in 25% of the samples tested with the molecular nucleic acid hybridization method and in 26% of the cases tested with the rapid antigen detection test. There was no significant difference between these three methods.
Keywords Acute pharyngotonsillitis
GABHS
laboratory detection
Language English
Date 2003-10-01
Published in Brazilian Journal of Infectious Diseases. Brazilian Society of Infectious Diseases, v. 7, n. 5, p. 297-300, 2003.
ISSN 1413-8670 (Sherpa/Romeo)
Publisher Brazilian Society of Infectious Diseases
Extent 297-300
Origin http://dx.doi.org/10.1590/S1413-86702003000500003
Access rights Open access Open Access
Type Article
SciELO ID S1413-86702003000500003 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/1885

Show full item record




File

Name: S1413-86702003000500003.pdf
Size: 20.11Kb
Format: PDF
Description:
Open file

This item appears in the following Collection(s)

Search


Browse

Statistics

My Account