Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae

Ascaris suum: partial fractionation of metabolic antigens from in vitro cultured larvae

Author Guerrero, Jorge Autor UNIFESP Google Scholar
Silverman, Paul H. Google Scholar
Institution Universidade Federal de São Paulo (UNIFESP)
University of New Mexico Department of Biology
Abstract Ascaris suum metabolic antigens were obtained frorn second and early third stage larvae cultured in vitro in supplemented Eagle's minimum essential medium. Metabolic antigens harvested after 12 and 16 days from in vitro cultures were eluted through Bio-Gel Al.5. Three main elution peaks were identified, dialysed, lyophilized and injected into mice with 4% sodium alginate. Peak 11 from elution of two preparations of metabolic antigens protected mice against a chállenge infection of 10,000 A. suum embryonated eggs.

Os antígenos metabólicos de Ascaris suum foram obtidos de larvas de segundo e de terceiro estágio (precoce) cultivadas in vitro em meio mínimo essencial de Eeagle com suplemento proteico. Os antígenos metabólicos coletados depois de 12 a 16 dias das culturas in vitro foram eluidos em Bio-Gel Al 5. Três picos de eluição foram identificados, dializados, liofilizados e injetados em camundongos, com alginato de sódio a 4%. O pico II da eluição de duas preparações dos antígenos metabólicos protegeram os camundongos da infecção com 10.000 ovos infectivos de A. suum
Language English
Date 1973-10-01
Published in Revista da Sociedade Brasileira de Medicina Tropical. Sociedade Brasileira de Medicina Tropical - SBMT, v. 7, n. 5, p. 319-327, 1973.
ISSN 0037-8682 (Sherpa/Romeo)
Publisher Sociedade Brasileira de Medicina Tropical - SBMT
Extent 319-327
Origin http://dx.doi.org/10.1590/S0037-86821973000500005
Access rights Open access Open Access
Type Article
SciELO ID S0037-86821973000500005 (statistics in SciELO)
URI http://repositorio.unifesp.br/handle/11600/154

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